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PMID: 6280155 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Differential stabilization by netropsin of inducible B-like conformations in deoxyribo-, ribo- and 2'-deoxy-2'-fluororibo-adenosine containing duplexes of (dA)n . (dT)n and (dA)n . (dU)na.

Nucleic acids research ·Vol. 10 ·No. 5 ·1982-03-11 ·Pages 1721-32

Zimmer C, Kakiuchi N, Guschlbauer W

Abstract

Six polynucleotide duplexes containing polydeoxyadenylic acid, polyadenylic acid or poly-2'-deoxy-2'-fluoro-adenylic acid in one strand, and polydeoxyuridylic acid or polydeoxythymidylic acid in the other strand have been studied by circular dichroism, ionic strength dependence of melting temperatures and binding of the DNA specific antibiotic netropsin. Circular dichroism spectra of (dA)n . (dT)n and (dA)n . (dU)n indicated the presence of the B-form of DNA, while those of (dAfl)n . (dT)n and (rA)n . (dT)n (and the corresponding (dU)n hybrids) indicated the presence of the A-form. (dAfl)n . (dT)n and (dAfl)n . (dU)n bound netropsin only slightly less than the (dA)n containing duplexes, while replacement by (rA)n decreased netropsin binding to a large degree. Since netropsin requires B-DNA for binding, it is concluded that the A to B transition is facilitated in the case of fluorine substitution in the sugar moiety, while the 2'-OH group greatly limits this conformational change.

MeSH Terms
Adenosine Circular Dichroism Floxuridine Guanidines Netropsin Nucleic Acid Conformation Polydeoxyribonucleotides Structure-Activity Relationship
Chemicals
Guanidines Polydeoxyribonucleotides Floxuridine Netropsin Adenosine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zimmer C
Kakiuchi N
Guschlbauer W
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.5.1721
Published
1982-03-11
Pages
1721-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320561
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.5.1721 PMC Full Text View journal details
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