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PMID: 6280176 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 4 ·1982-02-00 ·Pages 973-7

Patschinsky T, Hunter T, Esch FS, Cooper JA, Sefton BM

Abstract

We have identified the single phosphorylated tyrosine in p60src, the transforming protein of Rous sarcoma virus, as part of the sequence. NH2-Arg-Leu-Ile-Glu-Asp-Asn-Glu-Tyr(P)-Thr-Ala-Arg-COOH. Therefore, this is a sequence that is recognized efficiently by a tyrosine protein kinase in vivo. Phosphorylation of tyrosine in cellular proteins appears to play a role in malignant transformation by four classes of genetically distinct RNA tumor viruses. Phosphorylated tyrosines in several other proteins resemble of the tyrosine in p60src in that they are located 7 residues to the COOH-terminal side of a basic amino acid and either 4 residues to the COOH-terminal side of, or in close proximity to, a glutamic acid residue. Therefore it is possible that these features play a role in the selection of sites of phosphorylation by some tyrosine protein kinases. However, several clear exceptions to this rule exist.

MeSH Terms
Abelson murine leukemia virus Alpharetrovirus Amino Acid Sequence Avian Sarcoma Viruses Cell Transformation, Neoplastic Cell Transformation, Viral Immunoglobulin Heavy Chains Peptides/metabolism Phosphorylation Protein Kinases/metabolism Transforming Growth Factors Tyrosine/metabolism Viral Proteins/metabolism
Chemicals
Immunoglobulin Heavy Chains Peptides Viral Proteins Tyrosine Transforming Growth Factors Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Patschinsky T
Hunter T
Esch F S
Cooper J A
Sefton B M
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.4.973
Published
1982-02-00
Pages
973-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345881
Subset
IM
Grants
NCI NIH HHS · CA 14195 · United States
NCI NIH HHS · CA 17096 · United States
NCI NIH HHS · CA 17289 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.4.973 PMC Full Text View journal details
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