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PMID: 6283168 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Herpes simplex virus-induced changes in cellular and adenovirus RNA metabolism in an adenovirus type 5-transformed human cell line.

Journal of virology ·Vol. 42 ·No. 2 ·1982-05-00 ·Pages 474-87

Stenberg RM, Pizer LI

Abstract

We used the viral transcripts (designated Ad-RNA) that accumulated in the cytoplasm of adenovirus type 5-transformed human embryonic kidney cells (cell line 291-31) as models for cellular RNAs to examine how herpes simplex virus modifies cellular RNA metabolism. Infection of 293-31 cells with herpes simplex virus type 1 strain 17 lead to extensive inhibition of Ad-RNA accumulation by 4 h postinfection. The major part of this inhibition was due to an immediate early or alpha gene function, which reduced the rate of transcription of Ad-RNA within the nuclei of the infected cells. In addition, host polyadenylic acid-containing RNA accumulation and rRNA accumulation were affected, but to a lesser extent and at lower rate than Ad-RNA accumulation. In conjunction with previous data, our experimental data allowed us to propose a general scheme for how herpes simplex virus type 1 alters the metabolism of cellular RNA, the possible mechanisms for these changes, and how they correlate with the regulation of herpes simplex virus gene expression.

MeSH Terms
Adenoviruses, Human/genetics Cell Line Cell Transformation, Viral Embryo, Mammalian Humans Kidney RNA, Messenger/biosynthesis RNA, Ribosomal/biosynthesis RNA, Viral/biosynthesis Simplexvirus/genetics,physiology
Chemicals
RNA, Messenger RNA, Ribosomal RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stenberg R M
Pizer L I
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50 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.42.2.474-487.1982
Published
1982-05-00
Pages
474-87
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256874
Subset
IM
Grants
NIAID NIH HHS · 1R01 AI17873 · United States
NIAID NIH HHS · 5T32 AI07209 · United States
External Links
PubMed source DOI: 10.1128/JVI.42.2.474-487.1982 PMC Full Text View journal details
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