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PMID: 6283473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detailed structural analysis of two spliced HSV-1 immediate-early mRNAs.

Nucleic acids research ·Vol. 10 ·No. 7 ·1982-04-10 ·Pages 2241-56

Rixon FJ, Clements JB

Abstract

The structures of two HSV-1 immediate-early mRNAs have been determined by nuclease-digestion procedures using 5' and 3' end-labelled DNA probes. These mRNAs, which map across the junctions between the short unique (US) and short repeat (IRS and TRS) genome regions, have common 5' portions located in IRS and TRS. The 3' portions, which extend into opposite ends of US, and unique. The DNA sequence encoding the common 5' portions largely comprises a 247 base pair (bp) leader region and a single intron of variable size. The variation in intron length is due to different copy numbers of a 22 bp tandem reiteration. A small proportion of the mRNA population is unspliced, but otherwise is identical to the more abundant spliced species.

MeSH Terms
Animals Base Sequence Cell Line Cloning, Molecular Cricetinae DNA Restriction Enzymes Kidney Nucleic Acid Hybridization RNA Splicing RNA, Messenger/genetics Simplexvirus/genetics
Chemicals
RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rixon F J
Clements J B
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.7.2241
Published
1982-04-10
Pages
2241-56
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320606
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.7.2241 PMC Full Text View journal details
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