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PMID: 6285003 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and characterization of a group of discrete initiated oligonucleotides transcribed in vitro from the 3' terminus of the N-gene of vesicular stomatitis virus.

Journal of virology ·Vol. 42 ·No. 3 ·1982-06-00 ·Pages 889-96

Pinney DF, Emerson SU

Abstract

Four triphosphate-initiated oligonucleotides, 11 to 14 bases long, produced by in vitro transcription of vesicular stomatitis virus were identified as the uncapped 5' sequences of N-gene mRNA. Characterization of these oligonucleotides reveals that they are continually produced stable transcripts that do not remain template bound. Under the conditions used, the oligonucleotide transcripts were produced at 8 to 10 times the molar amount of leader, suggesting that the N-gene mRNA is internally initiated.

MeSH Terms
Base Sequence Genes, Viral Oligoribonucleotides/analysis,genetics RNA, Messenger/genetics RNA, Viral/genetics Transcription, Genetic Vesicular stomatitis Indiana virus/genetics
Chemicals
Oligoribonucleotides RNA, Messenger RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pinney D F
Emerson S U
References (31)
31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.42.3.889-896.1982
Published
1982-06-00
Pages
889-96
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256923
Subset
IM
Grants
NCI NIH HHS · 2T32-CA09109-06 · United States
NIAID NIH HHS · AI-11722 · United States
External Links
PubMed source DOI: 10.1128/JVI.42.3.889-896.1982 PMC Full Text View journal details
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