Home LiteratureArticle Details
PMID: 6285285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The anticodon of the maize chloroplast gene for tRNA Leu UAA is split by a large intron.

Nucleic acids research ·Vol. 10 ·No. 10 ·1982-05-25 ·Pages 3027-37

Steinmetz A, Gubbins EJ, Bogorad L

Abstract

The maize chloroplast gene encoding tRNA Leu UAA has been sequenced. It contains a 458 base pair intron between the first and second bases of the anticodon. The tRNA is 88 nucleotides long (the 3'-terminal CCA sequence included which, however, is not encoded by the gene) and differs in only four nucleotides (modified nucleotides are not considered) from the corresponding isoacceptor from bean chloroplasts. The unusual position of the intron in this maize chloroplast tRNA gene suggests a splicing model different from that generally accepted for eukaryotic split tRNA genes.

MeSH Terms
Anticodon/genetics Base Sequence Chloroplasts/analysis DNA/genetics DNA Restriction Enzymes Genes Nucleic Acid Conformation Plants/genetics RNA, Transfer/genetics RNA, Transfer, Amino Acyl/genetics Species Specificity Zea mays/genetics
Chemicals
Anticodon RNA, Transfer, Amino Acyl DNA RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Steinmetz A
Gubbins E J
Bogorad L
References (34)
34 references, click to expand
  1. Cytoplasmic site synthesis of chloroplast aminoacyl-tRNA synthetases in Euglena gracilis.
    FEBS Lett. 1973 Dec 15;38(1):70-4 PMID: 4204052
  2. Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes.
    Nature. 1980 Aug 28;286(5776):908-10 PMID: 6774264
  3. Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5487-91 PMID: 16592473
  4. Endonuclease recognition sites mapped on Zea mays chloroplast DNA.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4309-13 PMID: 16592373
  5. The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons.
    Cell. 1981 Nov;26(3 Pt 1):315-23 PMID: 6276012
  6. Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.
    Cell. 1980 Feb;19(2):345-53 PMID: 7357612
  7. Hybridization of maize chloroplast DNA with transfer ribonucleic acids.
    Biochemistry. 1976 Sep 7;15(18):4105-9 PMID: 963025
  8. Primary structure of three leucine transfer RNAs from bean chloroplast.
    Biochem Biophys Res Commun. 1980 Jan 15;92(1):102-8 PMID: 7356445
  9. The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.
    Nucleic Acids Res. 1982 Mar 11;10(5):1653-9 PMID: 6917975
  10. Compilation of tRNA sequences.
    Nucleic Acids Res. 1981 Jan 10;9(1):r1-23 PMID: 7208351
  11. Analysis of a drosophila tRNA gene cluster: two tRNALeu genes contain intervening sequences.
    Cell. 1981 Jan;23(1):251-9 PMID: 6783316
  12. Structure and processing of yeast precursor tRNAs containing intervening sequences.
    Nature. 1978 Aug 3;274(5670):438-45 PMID: 353564
  13. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5453-7 PMID: 341157
  14. The sites of transcription and translation for Euglena chloroplastic aminoacyl-tRNA synthetases.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1910-4 PMID: 4525469
  15. Transfer ribonucleic acid genes in the chloroplast deoxyribonucleic acid of pea leaves.
    Biochemistry. 1980 Dec 23;19(26):5973-81 PMID: 6907019
  16. Restriction endonuclease map of the chloroplast DNA of Chlamydomonas reinhardii.
    J Mol Biol. 1978 Dec 25;126(4):597-617 PMID: 745241
  17. Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes.
    Cell. 1981 Jul;25(1):203-13 PMID: 7023695
  18. The nucleotide sequences of two tRNAAsn genes from tobacco chloroplasts.
    Nucleic Acids Res. 1981 Nov 11;9(21):5601-7 PMID: 6273800
  19. Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6110-4 PMID: 16592597
  20. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  21. Isolation of yeast tRNALeu genes. DNA sequence of a cloned tRNALeu3 gene.
    J Biol Chem. 1979 Dec 25;254(24):12306-9 PMID: 387786
  22. In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence.
    Cell. 1979 Jun;17(2):399-406 PMID: 378410
  23. Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA.
    Gene. 1979 Aug;6(4):285-306 PMID: 499808
  24. Information content in the chloroplast DNA.
    Symp Soc Exp Biol. 1970;24:147-79 PMID: 4930349
  25. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  26. A precursor to a minor species of yeast tRNASer contains an intervening sequence.
    Cell. 1979 Sep;18(1):11-26 PMID: 389430
  27. Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the omega and rib-1 loci.
    Cell. 1980 May;20(1):185-97 PMID: 6156002
  28. Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):190-4 PMID: 343104
  29. An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi.
    Mol Gen Genet. 1974;132(2):119-29 PMID: 4421915
  30. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  31. Mendelian and uniparental alterations in erythromycin binding by plastid ribosomes.
    Science. 1971 Nov 12;174(4010):707-9 PMID: 5123420
  32. Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S--23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions.
    J Biol Chem. 1980 Nov 25;255(22):10997-1003 PMID: 6253497
  33. Transcription and processing of intervening sequences in yeast tRNA genes.
    Cell. 1978 Jun;14(2):221-36 PMID: 352537
  34. A gene coding for tRNA is located near 5' terminus of 16S rRNA gene in Zea mays chloroplast genome.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4748-52 PMID: 16593071
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.10.3027
Published
1982-05-25
Pages
3027-37
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320685
Subset
IM
Databases
GENBANK
K00635
External Links
PubMed source DOI: 10.1093/nar/10.10.3027 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2