Isopycnic separation of Escherichia coli cultures possessing colonization factor antigen I.

Journal of clinical microbiology ·Vol. 15 ·No. 6 ·1982-06-00 ·Pages 1074-6

Giesa FR, Zajac I, Bartus HF, Actor P

Abstract

A culture of Escherichia coli possessing colonization factor antigen I was subjected to isopycnic separation on Percoll gradients. The results demonstrated successful division of the culture into two populations: (i) bacteria which cause mannose-resistant hemagglutination and (ii) bacteria which lack the ability to hemagglutinate in the presence of mannose.

MeSH Terms

Antigens, Bacterial/analysis Centrifugation, Isopycnic Escherichia coli/immunology,isolation & purification Fimbriae Proteins Povidone Silicon Dioxide

Chemicals

Antigens, Bacterial colonization factor antigens Fimbriae Proteins Percoll Silicon Dioxide Povidone

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Giesa F R

Zajac I

Bartus H F

Actor P

References (11)

11 references, click to expand

Three characteristics associated with enterotoxigenic Escherichia coli isolated from man.
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Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans.
Infect Immun. 1975 Sep;12(3):656-67 PMID: 1100526
Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor.
Infect Immun. 1977 Nov;18(2):330-7 PMID: 336541
Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhea.
Infect Immun. 1978 Feb;19(2):727-36 PMID: 344221
Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose.
Infect Immun. 1979 Feb;23(2):336-46 PMID: 370014
A rapid single centrifugation step method for the separation of erythrocytes, granulocytes and mononuclear cells on continuous density gradients of Percoll.
J Immunol Methods. 1980;32(3):209-14 PMID: 7351486
Rapid separation of rat peritoneal mast cells with Percoll.
Eur J Cell Biol. 1980 Feb;20(3):272-5 PMID: 6244164
Synchronization of cell division in microorganisms by percoll gradients.
J Bacteriol. 1980 Oct;144(1):17-21 PMID: 6252189
Enterotoxigenic Escherichia coli and other gram-negative bacteria of infantile diarrhea: surface antigens, hemagglutinins, colonization factor antigen, and loss of enterotoxigenicity.
J Infect Dis. 1980 Sep;142(3):318-27 PMID: 7003030
Analysis of parameters affecting the hemagglutination activity of Escherichia coli possessing colonization factor antigens: improved medium for observing erythrocyte agglutination.
J Clin Microbiol. 1981 Feb;13(2):301-8 PMID: 7009637
Use of a hemadsorption technique to evaluate the stability of the hemagglutination reaction of Escherichia coli cultures possessing human colonization factor antigens.
J Clin Microbiol. 1982 Apr;15(4):554-7 PMID: 7040454

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.15.6.1074-1076.1982

Published

1982-06-00

Pages

1074-6

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC272256

Subset

External Links

PubMed source DOI: 10.1128/jcm.15.6.1074-1076.1982 PMC Full Text View journal details

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Isopycnic separation of Escherichia coli cultures possessing colonization factor antigen I.

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