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PMID: 6287223 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Syntheses and stabilities of proteins related to the polyoma small T antigen in Escherichia coli.

Molecular and cellular biology ·Vol. 2 ·No. 1 ·1982-01-00 ·Pages 88-92

Horwich A, Koop AH, Eckhart W

Abstract

We compared the syntheses and turnovers of two proteins related to the polyoma small T antigen synthesized in Escherichia coli from plasmids containing polyoma genomic segments joined to lac control elements. A protein with an authentic polyoma N terminus was more unstable than a protein with N-terminal amino acids derived from beta-galactosidase. Both were more unstable than most bacterial proteins.

MeSH Terms
Antigens, Viral/analysis,metabolism Antigens, Viral, Tumor Cloning, Molecular DNA Restriction Enzymes/pharmacology DNA, Recombinant Deoxyribonuclease EcoRI Escherichia coli/immunology,metabolism Operon Peptide Fragments Polyomavirus/immunology Viral Proteins/metabolism
Chemicals
Antigens, Viral Antigens, Viral, Tumor DNA, Recombinant Peptide Fragments Viral Proteins DNA Restriction Enzymes Deoxyribonuclease EcoRI
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Horwich A
Koop A H
Eckhart W
References (5)
5 references, click to expand
  1. A general method for maximizing the expression of a cloned gene.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):760-4 PMID: 370836
  2. Regions of the polyoma genome coding for T antigens.
    Nucleic Acids Res. 1979 Dec 20;7(8):2275-88 PMID: 230465
  3. Expression of the gene for the polyoma small T antigen in Escherichia coli.
    J Virol. 1980 Oct;36(1):125-32 PMID: 6255200
  4. Properties of simian virus 40 small t antigen overproduced in bacteria.
    J Virol. 1981 Feb;37(2):683-97 PMID: 6261013
  5. Expression of SV40 T antigen under control of adenovirus promoters.
    Cell. 1981 Mar;23(3):825-36 PMID: 7226231
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.1.88-92.1982
Published
1982-01-00
Pages
88-92
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369756
Subset
IM
Grants
NCI NIH HHS · CA 13884 · United States
NCI NIH HHS · CA14195 · United States
NIGMS NIH HHS · GM 25617 · United States
External Links
PubMed source DOI: 10.1128/mcb.2.1.88-92.1982 PMC Full Text View journal details
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