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PMID: 6289264 Published · ppublish English Journal Article

On the operon structure of the nitrogenase genes of Rhizobium leguminosarum and Azotobacter vinelandii.

Nucleic acids research ·Vol. 10 ·No. 14 ·1982-07-24 ·Pages 4147-57

Krol AJ, Hontelez JG, Roozendaal B, van Kammen A

Abstract

The transcription of the nitrogenase genes in Rhizobium leguminosarum was studied by analysing total cellular RNA from bacteroids for the presence of nitrogenase messenger RNA. The RNA was separated by agarose gel electrophoresis and blotted onto nitrocellulose filters. Messenger RNA for nitrogenase was detected by hybridization with probes derived from plasmid pSA30, a recombinant plasmid carrying the nitrogenase genes of Klebsiella pneumoniae. In the same way nitrogenase mRNA was detected in RNA isolated from Azotobacter vinelandii and from Klebsiella pneumoniae, but only if both were cultured under conditions of derepression of the nitrogenase genes. The size of the RNA hybridizing with the probes indicates that in all three organisms the genes for the subunits of the two components of nitrogenase constitute a single operon.

MeSH Terms
Azotobacter/enzymology,genetics DNA Restriction Enzymes Genes Klebsiella pneumoniae/enzymology Nitrogenase/genetics Nucleic Acid Hybridization Operon Plasmids RNA, Messenger/genetics Rhizobium/enzymology,genetics
Chemicals
RNA, Messenger Nitrogenase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Krol A J
Hontelez J G
Roozendaal B
van Kammen A
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18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.14.4147
Published
1982-07-24
Pages
4147-57
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320789
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.14.4147 PMC Full Text View journal details
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