Expression of large plasmids in the endosymbiotic form of Rhizobium leguminosarum.

Nucleic acids research ·Vol. 8 ·No. 19 ·1980-10-10 ·Pages 4337-47

Krol AJ, Hontelez JG, Van den Bos RC, Van Kammen A

Abstract

Isolated plasmid DNA from Rhizobium leguminosarum was hybridised with cellular RNA from broth-cultured bacteria and endosymbiotic bacteroids. From these hybridisation, experiments it is concluded that plasmid genes are strongly expressed in bacteroids and only weakly or not at all in bacteria. From the hybridisation of plasmid DNA with the cloned structural nif genes of Klebsiella pneumoniae it is shown that at least part of the nif genes are located on a plasmid.

MeSH Terms

Cloning, Molecular DNA, Recombinant/metabolism Genes Klebsiella pneumoniae/metabolism Molecular Weight Nucleic Acid Hybridization Plasmids Rhizobium/metabolism

Chemicals

DNA, Recombinant

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Krol A J

Hontelez J G

Van den Bos R C

Van Kammen A

References (12)

12 references, click to expand

A membrane-filter technique for the detection of complementary DNA.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
General method for the isolation of plasmid deoxyribonucleic acid.
J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
A freeze-squeeze method for recovering long DNA from agarose gels.
Anal Biochem. 1975 May 26;66(1):213-20 PMID: 1096670
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
The organization of genes in yeast mitochondrial DNA II. The physical map of EcoRI and HindII + III fragments.
Mol Gen Genet. 1975 Dec 30;143(1):53-64 PMID: 765743
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by Rhizobium leguminosarum.
Biochim Biophys Acta. 1978 Feb 13;539(1):1-11 PMID: 623788
IncP2 group of Pseudomonas, a class of uniquely large plasmids.
Nature. 1978 Aug 17;274(5672):715-7 PMID: 673007
[Demonstration of extrachromosomal DNA in Rhizobium meliloti].
Can J Microbiol. 1978 Aug;24(8):960-6 PMID: 688104
The organization of ribosomal RNA genes in the mitochondrial DNA of Tetrahymena pyriformis strain ST.
Biochim Biophys Acta. 1978 Nov 21;521(1):169-86 PMID: 102353
Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized.
Mol Gen Genet. 1979 Jul 2;174(1):59-66 PMID: 384162
Interspecies homology of nitrogenase genes.
Proc Natl Acad Sci U S A. 1980 Jan;77(1):191-5 PMID: 6987649

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.19.4337

Published

1980-10-10

Pages

4337-47

Language

English

Region

England

NLM ID

0411011

PMCID

PMC324243

Subset

External Links

PubMed source DOI: 10.1093/nar/8.19.4337 PMC Full Text View journal details

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Expression of large plasmids in the endosymbiotic form of Rhizobium leguminosarum.

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