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PMID: 6290450 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular mapping of glyW, a duplicate gene for tRNA3Gly of Escherichia coli.

Journal of bacteriology ·Vol. 152 ·No. 2 ·1982-11-00 ·Pages 773-9

Tucker SD, Gopalakrishnan AS, Bollinger R, Dowhan W, Murgola EJ

Abstract

By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order of genes in this region is uvrC, pgsA, glyW, flaI. Comparison of the order of determined restriction sites with the sites predicted from the nucleotide sequence of tRNA3Gly indicates that the direction of transcription of glyW is counterclockwise on the circular E. coli map.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Bacterial/physiology Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genes, Bacterial Nucleic Acid Hybridization Plasmids RNA, Transfer, Amino Acyl/genetics
Chemicals
RNA, Transfer, Amino Acyl DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tucker S D
Gopalakrishnan A S
Bollinger R
Dowhan W
Murgola E J
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21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.2.773-779.1982
Published
1982-11-00
Pages
773-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221528
Subset
IM
Grants
NCI NIH HHS · CA-09299 · United States
NIGMS NIH HHS · GM 21499 · United States
NIGMS NIH HHS · GM 25047 · United States
Databases
GENBANK
J01624
External Links
PubMed source DOI: 10.1128/jb.152.2.773-779.1982 PMC Full Text View journal details
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