Sequence variation and methylation of the flax 5S RNA genes.

Nucleic acids research ·Vol. 10 ·No. 15 ·1982-08-11 ·Pages 4501-14

Goldsbrough PB, Ellis TH, Lomonossoff GP

Abstract

The complete sequence of the flax 5S DNA repeat is presented. Length heterogeneity is the consequence of the presence or absence of a single direct repeat and the majority of single base changes are transition mutations. No sequence variation has been found in the coding sequence. The extent of methylation of cytosines has been measured at one location in the gene and one in the spacer. The relationship between the observed sequence heterogeneity and the level of methylation is discussed in the context of the operation of a correction mechanism.

MeSH Terms

DNA/genetics DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Methylation Molecular Weight Nucleic Acid Hybridization Plants/genetics Plasmids RNA, Ribosomal/genetics Repetitive Sequences, Nucleic Acid Species Specificity Triticum

Chemicals

DNA, Recombinant RNA, Ribosomal DNA DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Goldsbrough P B

Ellis T H

Lomonossoff G P

References (17)

17 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.15.4501

Published

1982-08-11

Pages

4501-14

Language

English

Region

England

NLM ID

0411011

PMCID

PMC321107

Subset

Databases

GENBANK

K03148, K03149, K03150, K03151, X01531

External Links

PubMed source DOI: 10.1093/nar/10.15.4501 PMC Full Text View journal details

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Sequence variation and methylation of the flax 5S RNA genes.

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