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PMID: 6291057 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Human antibody to OFA-I, a tumor antigen, produced in vitro by Epstein-Barr virus-transformed human B-lymphoid cell lines.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 18 ·1982-09-00 ·Pages 5666-70

Irie RF, Sze LL, Saxton RE

Abstract

We established two long-term human B-lymphoblastoid cell lines (L55 and L72) transformed by Epstein-Barr virus that produced IgM kappa antibodies to the human tumor antigen, OFA-I. Periphral blood lymphocytes obtained from melanoma patients were used as the source of the B lymphocytes. Antibody specificity was determined by the immune adherence assay using various human cancer and noncancer tissues as targets. L55 antibody (designated anti-OFA-I-1) reacted with a variety of human tumor types whereas L72 antibody (designated anti-OFA-I-2) reacted only with tumor cells of neuroectodermal origin (melanoma, glioma, and neuroblastoma). The levels of IgM detected in the spent medium of 1 X 10(6) L55 and L72 cells were 4 and 9 micrograms/ml, respectively, by radioimmunoassay.

MeSH Terms
Antibody Formation Antigens, Neoplasm/genetics B-Lymphocytes/immunology Burkitt Lymphoma/immunology,microbiology Cell Line Cell Transformation, Neoplastic Herpesvirus 4, Human/genetics,immunology Humans Immunoglobulin M/genetics Immunoglobulin kappa-Chains/genetics Melanoma/immunology Neoplasms/immunology
Chemicals
Antigens, Neoplasm Immunoglobulin M Immunoglobulin kappa-Chains oncofetal antigens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Irie R F
Sze L L
Saxton R E
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.18.5666
Published
1982-09-00
Pages
5666-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346965
Subset
IM
Grants
NCI NIH HHS · CA00543 · United States
NCI NIH HHS · R01 CA30647 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.18.5666 PMC Full Text View journal details
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