Home LiteratureArticle Details

PMID: 80012 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Study of antibodies against human melanoma produced by somatic cell hybrids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 7 ·1978-07-00 ·Pages 3405-9

Koprowski H, Steplewski Z, Herlyn D, Herlyn M

Abstract

We fused spleen lymphocytes obtained from mice immunized against a human melanoma cell line and melanoma-mouse hybrid cells with the P3 X 63 Ag8 mouse myeloma in order to produce hybrids secreting antibodies against a human melanoma. Antibodies secreted by individual hybrids were tested for their reaction with a panel of human melanoma, colorectal carcinoma, and normal cells in an indirect radioimmunoassay, and they displayed different specificities and crossreactivities. Some reacted only with melanomas, whereas others crossreacted with normal human or human colorectal carcinoma cells. By analysis of competitive binding of mixtures of monoclonal antibodies, it was possible to delineate different epitopes on melanomas. Hybrids growing in nude mice and producing antimelanoma antibody suppressed growth of melanoma tumors.

MeSH Terms

Animals Antibodies, Neoplasm/biosynthesis Antibody Specificity Carcinoma/immunology Cell Line Clone Cells/immunology Colonic Neoplasms/immunology Cross Reactions Epitopes Humans Hybrid Cells/immunology Melanoma/immunology Mice Rectal Neoplasms/immunology Spleen/immunology

Chemicals

Antibodies, Neoplasm Epitopes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Koprowski H

Steplewski Z

Herlyn D

Herlyn M

References (12)

12 references, click to expand

Classification of human colorectal adenocarcinoma cell lines.
Cancer Res. 1976 Dec;36(12):4562-9 PMID: 1000501
Continuous cultures of fused cells secreting antibody of predefined specificity.
Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.
Science. 1964 Aug 14;145(3633):709-10 PMID: 14168277
Alteration of cell surfaces with increased tumorigenicity.
Arch Immunol Ther Exp (Warsz). 1977;25(3):331-9 PMID: 196567
Repertoire of antiviral antibodies expressed by somatic cell hybrids.
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1510-4 PMID: 206903
Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8 PMID: 268647
The complete amino acid sequence of a mouse kappa light chain.
Biochem J. 1972 Jun;128(2):427-44 PMID: 4638343
A microtest radioimmunoassay for noncytotoxic tumor-specific antibody to cell-surface antigens.
J Natl Cancer Inst. 1973 Nov;51(5):1713-5 PMID: 4762940
Genetic analysis with man-mouse somatic cell hybrids. Linkage between human lactate dehydrogenase B and peptidase B genes.
Nature. 1970 Jul 18;227(5255):248-51 PMID: 5428190
Physical properties of human interferon prepared in vitro and in vivo.
Virology. 1966 Aug;29(4):515-22 PMID: 5945709
Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40.
Proc Natl Acad Sci U S A. 1978 May;75(5):2320-3 PMID: 79181
Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.
Eur J Immunol. 1976 Jul;6(7):511-9 PMID: 825377

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.7.3405

Published

1978-07-00

Pages

3405-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392785

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.7.3405 PMC Full Text View journal details

Analysis Services

Study of antibodies against human melanoma produced by somatic cell hybrids.

Contact

WeChat Official Account