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PMID: 79181 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 5 ·1978-05-00 ·Pages 2320-3

Martinis J, Croce CM

Abstract

We have produced somatic cell hybrids between mouse myeloma cells deficient in hypoxanthine phosphoribosyltransferase IMP: pyrophosphate phosphoribosyltransferase; EC 2.4.2.8) and spleen cells derived from mice primed with either syngeneic or allogeneic cells transformed by simian virus 40. Such hybrids produced antibodies specific for simian virus 40 tumor (T) antigen. Only four of twelve independent hybrid cell cultures produced antibodies against simian virus 40 T antigen that crossreacted with the T antigen induced by BK virus, a human papovavirus isolated from patients who had undergone immunosuppressive therapy.

MeSH Terms

Animals Antibodies, Neoplasm/biosynthesis Antibodies, Viral/biosynthesis Antibody Specificity BK Virus/immunology Clone Cells/immunology Cross Reactions Epitopes Hybrid Cells/immunology Mice Myeloma Proteins/immunology Polyomavirus/immunology Simian virus 40/immunology Spleen/immunology

Chemicals

Antibodies, Neoplasm Antibodies, Viral Epitopes Myeloma Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Martinis J

Croce C M

References (12)

12 references, click to expand

A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS.
Proc Natl Acad Sci U S A. 1963 Dec;50:1148-56 PMID: 14096190
SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.
Science. 1964 Aug 14;145(3633):709-10 PMID: 14168277
DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE.
J Exp Med. 1964 Aug 1;120:121-8 PMID: 14206435
Malignant transformation of hamster kidney cells by BK virus.
J Virol. 1975 Feb;15(2):420-2 PMID: 163364
Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):457-61 PMID: 191809
Organization and expression of early genes of simian virus 40.
Proc Natl Acad Sci U S A. 1978 Jan;75(1):117-21 PMID: 203926
Spliced early mRNAs of simian virus 40.
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
Repertoire of antiviral antibodies expressed by somatic cell hybrids.
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1510-4 PMID: 206903
Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8 PMID: 268647
New human papovavirus (B.K.) isolated from urine after renal transplantation.
Lancet. 1971 Jun 19;1(7712):1253-7 PMID: 4104714
Antibodies to major histocompatibility antigens produced by hybrid cell lines.
Nature. 1977 Apr 7;266(5602):550-2 PMID: 558524
Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.
Eur J Immunol. 1976 Jul;6(7):511-9 PMID: 825377

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.5.2320

Published

1978-05-00

Pages

2320-3

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392544

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.5.2320 PMC Full Text View journal details

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Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40.

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