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PMID: 6292446 Published · ppublish English Journal Article

In vitro transcription of two human rotaviruses.

Journal of virology ·Vol. 43 ·No. 3 ·1982-09-00 ·Pages 1032-7

Flores J, Myslinski J, Kalica AR, Greenberg HB, Wyatt RG, Kapikian AZ, Chanock RM

Abstract

The RNA polymerase activities of a cultivatable (Wa) and a noncultivatable (DS-1) strain of human rotavirus were studied. Under optimal conditions, transcription of all of their RNA segments occurred, as evidenced by the hybridization of labeled transcripts to genomic RNA. Cross-hybridization between the two viruses showed that none of their 11 genes were completely homologous. The transcription products could be translated in vitro, yielding proteins with an electrophoretic pattern resembling that obtained with proteins labeled in vivo during infection with the Wa virus.

MeSH Terms
Cell-Free System Child Humans Nucleic Acid Hybridization Protein Biosynthesis RNA Nucleotidyltransferases/metabolism RNA, Double-Stranded/metabolism RNA, Viral/metabolism RNA-Dependent RNA Polymerase/metabolism Rotavirus/enzymology,genetics Transcription, Genetic Viral Proteins/metabolism
Chemicals
RNA, Double-Stranded RNA, Viral Viral Proteins RNA Nucleotidyltransferases RNA-Dependent RNA Polymerase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Flores J
Myslinski J
Kalica A R
Greenberg H B
Wyatt R G
Kapikian A Z
Chanock R M
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23 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.43.3.1032-1037.1982
Published
1982-09-00
Pages
1032-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256214
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.43.3.1032-1037.1982 PMC Full Text View journal details
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