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PMID: 6292464 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Simian virus 40 encapsidation: characterization of early intermediates.

Journal of virology ·Vol. 43 ·No. 3 ·1982-09-00 ·Pages 830-9

Milavetz B, Hopkins T

Abstract

Simian virus 40 chromosomes were separated into various species by a two-step purification consisting of low-ionic-strength glycerol gradient sedimentation followed by low-ionic-strength agarose gel electrophoresis. For each species of simian virus 40 chromosome purified, the comigrating DNA and proteins were identified by agarose or polyacrylamide gel electrophoresis, respectively. Two species of chromosomes were identified which contained form I and form II DNA and large amounts of viral protein; they migrated more slowly than most of the free simian virus 40 chromosomes, which contained very little viral protein. The nuclease susceptibility of these chromosomes suggests to us that they are intermediates in encapsidation, and we describe an encapsidation model.

MeSH Terms
Capsid/metabolism DNA, Viral/isolation & purification Deoxyribonucleases Deoxyribonucleoproteins/isolation & purification Electrophoresis, Agar Gel Morphogenesis Simian virus 40/metabolism,ultrastructure Viral Proteins/isolation & purification,metabolism
Chemicals
DNA, Viral Deoxyribonucleoproteins Viral Proteins Deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Milavetz B
Hopkins T
References (54)
54 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.43.3.830-839.1982
Published
1982-09-00
Pages
830-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256193
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.43.3.830-839.1982 PMC Full Text View journal details
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