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PMID: 6292500 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma.

Journal of virology ·Vol. 44 ·No. 1 ·1982-10-00 ·Pages 393-400

Gissmann L, Diehl V, Schultz-Coulon HJ, zur Hausen H

Abstract

Papilloma virus DNA from a laryngeal papilloma was cloned in phage lambda L 47 and characterized after cleavage with different restriction enzymes. Hybridization with the DNAs of human papilloma virus types 1, 2, 3, 4, 5, and 8 showed no homology under stringent hybridization conditions. Human papilloma virus type 6 DNA, however, was partially identical to laryngeal papilloma virus DNA; different restriction enzyme fragments hybridizing with the other DNA were identified on each genome. The degree of homology was determined by reassociation kinetics to be 25%. According to the present nomenclature, laryngeal papilloma virus therefore represents a different type of human papilloma virus and is tentatively designated as human papilloma virus type 11. Sequences homologous to laryngeal papilloma virus DNA were also found in four of nine additional laryngeal papillomas. Attempt to detect homologous DNA in 12 carcinomas of the larynx were negative.

MeSH Terms
Adolescent Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Viral Female Humans Laryngeal Neoplasms/microbiology Nucleic Acid Hybridization Papilloma/microbiology Papillomaviridae/classification,genetics Tumor Virus Infections/microbiology
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gissmann L
Diehl V
Schultz-Coulon H J
zur Hausen H
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27 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.44.1.393-400.1982
Published
1982-10-00
Pages
393-400
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256274
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.44.1.393-400.1982 PMC Full Text View journal details
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