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PMID: 6294345 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional analysis of a simian virus 40 super T-antigen.

Journal of virology ·Vol. 44 ·No. 3 ·1982-12-00 ·Pages 974-82

Clayton CE, Lovett M, Rigby PW

Abstract

The SV3T3 C120 line of simian virus 40-transformed mouse cells synthesizes no large T-antigen of molecular weight 94,000 but instead a super T-antigen of molecular weight 145,000. In the accompanying paper (Lovett et al., J. Virol. 44:963-973, 1982), we showed that the integrated viral DNA segment SV3T3-20-K contains a perfect, in-phase, tandem duplication of 1.212 kilobases within the large T-antigen coding sequences. Our data suggested that this integrated template encodes mRNAs of 3.9 and 3.6 kilobases, the smaller of which directs the synthesis of the super T-antigen of molecular weight 145,000. We transfected the DNA segment SV3T3-20-K into nonpermissive rat cells and into TK- mouse L cells and analyzed the T-antigens and viral mRNAs in the transfectants; these data prove directly the coding assignments suggested previously. The super T-antigen retained the ability to induce morphological transformation, and may even transform better than the wild-type protein. It also retained the ability to bind to the cell-coded p53 protein. Transfection into permissive CV-1 cells showed that the super T-antigen encoded by SV3T3-20-K was incapable of initiating DNA replication at the viral origin. The duplication in SV3T3-20-K thus defines a mutation which separates the transformation and DNA replication functions of large T-antigen. We discuss why such mutations may be selected in transformed cells.

MeSH Terms
Animals Antigens, Viral/genetics,isolation & purification Antigens, Viral, Tumor Cell Line Cell Transformation, Neoplastic DNA Restriction Enzymes DNA, Viral/genetics Immunoenzyme Techniques Nucleic Acid Hybridization RNA, Viral/genetics Rats Simian virus 40/immunology Templates, Genetic Transfection
Chemicals
Antigens, Viral Antigens, Viral, Tumor DNA, Viral RNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clayton C E
Lovett M
Rigby P W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.44.3.974-982.1982
Published
1982-12-00
Pages
974-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256357
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.44.3.974-982.1982 PMC Full Text View journal details
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