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PMID: 6294465 Published · ppublish English Journal Article

Sequence relationships between plasmids associated with conventional MLS resistance and zonal lincomycin resistance in Streptococcus pyogenes.

Molecular & general genetics : MGG ·Vol. 187 ·No. 2 ·1982-00-00 ·Pages 310-5

Golubkov VI, Reichardt W, Boitsov AS, Iontova IM, Malke H, Totolian AA

Abstract

By using electron microscopy of self-annealed DNA and restriction enzyme analysis, we have compared the physical maps of two group A streptococcal plasmids associated with conventional MLS resistance (pEL1; 20 Md) and zonal lincomycin resistance (pSM10419; 15 Md). Of their monomeric molecules, about 40% and 60%, respectively, are occupied by identical non-tandem inverted repeats containing sequences specifying putative replication functions. Sequence homology also exists between their resistance determinants which are located in unique DNA. Moreover, homology between additional regions of unknown function is so extensive and restriction fragment arrangement so similar that, formally, pSM10419 can be considered a deletion variant of pEL1. The results suggest that MLS and zonal lincomycin resistance have the same biochemical basis (i.e. methylation of 23S ribosomal RNA) and differ only quantitatively in the inducible control systems.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Bacterial DNA, Recombinant Drug Resistance, Microbial Lincomycin/pharmacology Microscopy, Electron Plasmids Repetitive Sequences, Nucleic Acid Streptococcus pyogenes/genetics,metabolism Virginiamycin/pharmacology
Chemicals
DNA, Bacterial DNA, Recombinant Virginiamycin Lincomycin DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Golubkov V I
Reichardt W
Boitsov A S
Iontova I M
Malke H
Totolian A A
References (16)
16 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331135
Published
1982-00-00
Pages
310-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331135
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