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PMID: 6294613 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Ribosomal RNA genes of Trypanosoma brucei. Cloning of a rRNA gene containing a mobile element.

Nucleic acids research ·Vol. 10 ·No. 21 ·1982-11-11 ·Pages 6747-61

Hasan G, Turner MJ, Cordingley JS

Abstract

An ordered restriction map of the ribosomal RNA genes of Trypanosoma brucei brucei is presented. Bgl II fragments of T.b.brucei genomic DNA were cloned into pAT 153, and the clones containing rDNA identified. Restriction maps were established and the sense strands identified. One clone was shown by heteroduplex mapping to contain a 1.1 kb inserted sequence which was demonstrated to be widely distributed throughout the genomes of members of the subgenus Trypanozoon. However, in two other subgenera of Trypanosoma, Nannomonas and Schizotrypanum, the sequence is far less abundant. Analysis of the genomic DNA from two serodemes of T.b.brucei showed that the sequence was present in the rRNA of only one of them, implying that the sequence is a mobile element and that its appearance in rDNA is a comparitively recent occurrence.

MeSH Terms

Animals Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA Transposable Elements DNA, Ribosomal Genes Microscopy, Electron Nucleic Acid Hybridization RNA, Ribosomal/genetics Species Specificity Trypanosoma brucei brucei/genetics

Chemicals

DNA Transposable Elements DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hasan G

Turner M J

Cordingley J S

References (12)

12 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.21.6747

Published

1982-11-11

Pages

6747-61

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326961

Subset

External Links

PubMed source DOI: 10.1093/nar/10.21.6747 PMC Full Text View journal details

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Ribosomal RNA genes of Trypanosoma brucei. Cloning of a rRNA gene containing a mobile element.

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