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PMID: 6294652 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Insulin activates a tyrosine-specific protein kinase in extracts of 3T3-L1 adipocytes and human placenta.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 22 ·1982-11-00 ·Pages 6792-6

Petruzzelli LM, Ganguly S, Smith CJ, Cobb MH, Rubin CS, Rosen OM

Abstract

Insulin activates a tyrosine-specific cAMP-independent protein kinase when added directly to detergent extracts of differentiated 3T3-L1 adipocytes and humal placental membranes. The kinase is also activated by antibody to the insulin receptor and, to a lesser extent, by proinsulin. It catalyzes the phosphorylation of the 92,000-dalton component of the insulin receptor, histone, and casein; in each case, tyrosine is the principal amino acid modified. Under the conditions used to activate the kinase, insulin does not affect the rate of dephosphorylation of the receptor or of histone. The insulin-activated kinase is copurified with the human placental insulin receptor until the final elution from insulin-Sepharose. It remains to be established whether the kinase and the insulin receptor are separate molecules.

MeSH Terms
Adipose Tissue/drug effects,enzymology Animals Cells, Cultured Female Humans Insulin/pharmacology Kinetics Mice Molecular Weight Phosphorylation Placenta/drug effects,enzymology Pregnancy Protamine Kinase/metabolism Protein Kinases/metabolism Receptor, Insulin/drug effects,metabolism Substrate Specificity Tyrosine
Chemicals
Insulin Tyrosine Protein Kinases Receptor, Insulin Protamine Kinase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Petruzzelli L M
Ganguly S
Smith C J
Cobb M H
Rubin C S
Rosen O M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.22.6792
Published
1982-11-00
Pages
6792-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347219
Subset
IM
Grants
NIADDK NIH HHS · AM-09038 · United States
NIADDK NIH HHS · AM-21248 · United States
NIGMS NIH HHS · GM-7288 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.22.6792 PMC Full Text View journal details
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