Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.

Nucleic acids research ·Vol. 10 ·No. 22 ·1982-11-25 ·Pages 7439-48

Kohli V, Balland A, Wintzerith M, Sauerwald R, Staub A, Lecocq JP

Abstract

The phosphotriester method for the stepwise synthesis of deoxyoligonucleotides has been employed using HPLC-grade silica gel (Porasil B) as the solid support. The procedure results in a convenient flow-through system for the synthesis of oligomers where all the reaction steps including the zinc bromide method of detritylation are compatible with the selected support. Deoxyoligonucleotides of 25-30 nucleotides in length can be synthesized in high yields utilising stable phosphotriester intermediates. Ease of handling of the solid support allows convenient synthesis of mixed oligonucleotide sequences.

MeSH Terms

Base Sequence Chromatography, High Pressure Liquid Indicators and Reagents Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Organophosphates Silica Gel Silicon Dioxide

Chemicals

Indicators and Reagents Oligodeoxyribonucleotides Oligonucleotides Organophosphates Silica Gel Silicon Dioxide

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kohli V

Balland A

Wintzerith M

Sauerwald R

Staub A

Lecocq J P

References (13)

13 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.22.7439

Published

1982-11-25

Pages

7439-48

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327016

Subset

External Links

PubMed source DOI: 10.1093/nar/10.22.7439 PMC Full Text View journal details

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Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.

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