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PMID: 6296846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Host range mutants of vesicular stomatitis virus defective in in vitro RNA methylation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 24 ·1982-12-00 ·Pages 7694-8

Horikami SM, Moyer SA

Abstract

The viral RNA polymerase of detergent-treated vesicular stomatitis virus normally synthesizes viral mRNAs in vitro that are both guanylylated and methylated to give 5'-terminal 7mGpppAm caps. We have characterized a virus host range mutant, hr 1, that is totally defective in vitro in the methylation of mRNA, although full-length polyadenylylated mRNAs with 5' termini of the form GpppA are synthesized in normal yields. A second mutant, hr 8, is partially defective in methylation and synthesizes mRNAs in vitro with primarily GpppA and some GpppAm 5' termini. When used for in vitro translation, the unmethylated hr 1 mutant mRNA shows, as expected, reduced synthesis of viral proteins. These data provide direct evidence that the vesicular stomatitis virus-associated methyltransferase activities are virus encoded.

MeSH Terms
Cell-Free System Genes, Viral Methylation Methyltransferases/genetics Mutation Protein Biosynthesis RNA, Messenger/genetics Vesicular stomatitis Indiana virus/genetics Virus Replication
Chemicals
RNA, Messenger Methyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Horikami S M
Moyer S A
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.24.7694
Published
1982-12-00
Pages
7694-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347414
Subset
IM
Grants
NIAID NIH HHS · AI 14594 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.24.7694 PMC Full Text View journal details
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