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PMID: 6298577 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterisation of the promoters for the ompA gene which encodes a major outer membrane protein of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 188 ·No. 3 ·1982-00-00 ·Pages 472-9

Cole ST, Bremer E, Hindennach I, Henning U

Abstract

The regulatory region of the ompA gene from Escherichia coli has been characterized by biochemical and genetic approaches. Two overlapping promoters, P1 and P2, organized in that order with respect to the ompA coding sequence, were identified and it was found that ompA possesses an unusually long leader region. Both P1 and P2 were active in an in vitro transcription system although S1 mapping analysis of the ompA mRNA made in vivo showed that P2 was mainly responsible for transcription of the gene. Confirmation of this was obtained by studying down-promoter mutants of ompA cloned in pSC101. These mutants were classified into two groups, deletions and insertions. The deletions, which were caused by the IS102 insertion element found in pSC101 removed the--35 regions of both P1 and P2. However, since P2 was distally situated with respect to the IS element it was less extensively damaged and it is proposed that the residual P2 sequence is responsible for the low level of expression observed. In addition to an IS102 insertion in the promoter region four IS1 insertion mutants were characterized. These had integrated at different positions in the ompA leader region and were all incompletely polar.

MeSH Terms
Bacterial Outer Membrane Proteins Bacterial Proteins/genetics Base Sequence DNA Restriction Enzymes Genes Genes, Bacterial Membrane Proteins/genetics Mutation Operon Plasmids Species Specificity Transcription, Genetic
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Membrane Proteins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cole S T
Bremer E
Hindennach I
Henning U
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49 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330051
Published
1982-00-00
Pages
472-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330051
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