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PMID: 6298702 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The organization of the tadpole and adult alpha globin genes of Xenopus laevis.

Nucleic acids research ·Vol. 10 ·No. 24 ·1982-12-20 ·Pages 7935-45

Patient RK, Banville D, Brewer AC, Elkington JA, Greaves DR, Lloyd MM, Williams JG

Abstract

Adult erythrocytes of X. laevis contain six electrophoretically resolvable globin polypeptides while tadpole erythrocytes contain four polypeptides, none of which comigrates with an adult protein. We show that three of the adult proteins are alpha globin polypeptides (alpha 1, alpha 2, alpha 3) and three are beta globin polypeptides (beta 1, beta 2, beta 3). We find that a tadpole alpha globin gene (alpha T1) is linked to the major adult locus in the sequence 5'-alpha T1-alpha 1-beta 1-3' with 5.2 kb separating alpha T1 from alpha 1. Another tadpole alpha globin gene (alpha T2) is linked to the minor adult locus in the sequence 5'-alpha T2-alpha 2-beta 2-3' with 10.7 kb separating alpha T2 from alpha 2. These linkage relationships are consistent with the major and minor loci having arisen by tetraploidization but the different separation of larval and adult globin genes at the two loci indicates the occurrence of some additional chromosomal rearrangement. Two alternative models are presented.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes DNA, Recombinant/metabolism Erythrocytes/metabolism Genes Globins/genetics Metamorphosis, Biological Nucleic Acid Hybridization Peptides/isolation & purification Protein Biosynthesis Xenopus
Chemicals
DNA, Recombinant Peptides Globins DNA DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Patient R K
Banville D
Brewer A C
Elkington J A
Greaves D R
Lloyd M M
Williams J G
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.24.7935
Published
1982-12-20
Pages
7935-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327060
Subset
IM
Databases
GENBANK
J00976
External Links
PubMed source DOI: 10.1093/nar/10.24.7935 PMC Full Text View journal details
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