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PMID: 6300424 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Recombinational junctions of variants of Moloney murine sarcoma virus: generation and divergence of a mammalian transforming gene.

Journal of virology ·Vol. 45 ·No. 2 ·1983-02-00 ·Pages 607-17

Donoghue DJ, Hunter T

Abstract

Different variants of Moloney murine sarcoma virus (MSV) were examined by nucleotide sequencing to compare the junctions between the acquired cellular sequence, v-mos, and the adjacent virus-derived sequences. These variants included 124-MSV, m1-MSV, and HT1-MSV and also the purportedly independent isolate Gazdar MSV. These four strains have an identical 5' junction between the murine leukemia virus env gene and the v-mos gene. This junction lies within the sixth codon of the chimeric env-mos coding region that encodes the transforming gene product. In contrast, at the 3' junction between the v-mos gene and the murine leukemia virus env gene, the three variants examined here were all different. A small deletion was found in the COOH-terminal portion of the m1-MSV env-mos coding region, indicating that the COOH terminus of this transforming gene product must be different from that of 124-MSV or HT1-MSV. The data presented here are consistent with the thesis that a virus closely related to HT1-MSV was the primordial Moloney MSV, and that all other related strains evolved from it by deletion or rearrangement. The variability observed in the Moloney MSV family is discussed in terms of possible mechanisms for the initial capture of mos sequences by the parental retrovirus and also in comparison with other transforming retrovirus families, such as Abelson murine leukemia virus and Rous sarcoma virus.

MeSH Terms
Animals Base Sequence Cell Transformation, Viral Codon Defective Viruses/genetics Mice Mice, Inbred NZB/genetics Oncogenes Recombination, Genetic Sarcoma Viruses, Murine/genetics Viral Envelope Proteins Viral Proteins/genetics
Chemicals
Codon Viral Envelope Proteins Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Donoghue D J
Hunter T
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44 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.45.2.607-617.1983
Published
1983-02-00
Pages
607-17
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256455
Subset
IM
Grants
NCI NIH HHS · CA-14195 · United States
NCI NIH HHS · CA-17096 · United States
NCI NIH HHS · CA-28458 · United States
Databases
GENBANK
J00371, J00372, K03105, K03106, K03107, K03108, K03109
External Links
PubMed source DOI: 10.1128/JVI.45.2.607-617.1983 PMC Full Text View journal details
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