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PMID: 159456 Published · ppublish English Journal Article

Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4464-8

Vande Woude GF, Oskarsson M, Enquist LW, Nomura S, Sullivan M, Fischinger PJ

Abstract

We have identified integrated proviral DNA sequences of m1 and HT-1 isolates of Moloney sarcoma virus (MuSV) in EcoRI digests of transformed mink cell genomic DNA and have cloned these fragments in bacteriophage lambda. Both the lambda-HT1 phage recombinant, containing a 12.3-kilobase MuSV pair (kb) fragment, and the lambda-m1 phage recombinant, containing a 7.0-kb fragment, possess full copies of the sarcoma viruses along with 5' and 3' host flanking sequences. The MuSV proviral DNA sequences, 6.7 kb for HT-1 and 5.2 kb for m1, are colinear by heteroduplex microscopy with the 1.5-kb difference in size accounted for by two approximately equal to 0.8-kb deleted regions in m1. Both integrated viral genomes are terminally redundant and have integrated at the same site in the provirus but at different sites on the host chromosome. The host sequence flanking integrated HT-1 MuSV have been identified as a single EcoRI restriction fragment of 5.6 kb in normal mink cells.

MeSH Terms
Animals Bacteriophage lambda/genetics Cells, Cultured DNA Restriction Enzymes/metabolism DNA, Recombinant DNA, Viral/genetics Genes, Viral Mink Moloney murine leukemia virus/genetics Recombination, Genetic
Chemicals
DNA, Recombinant DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vande Woude G F
Oskarsson M
Enquist L W
Nomura S
Sullivan M
Fischinger P J
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4464
Published
1979-09-00
Pages
4464-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411597
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4464 PMC Full Text View journal details
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