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PMID: 178891 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Deletion mapping of moloney type C virus: polypeptide and nucleic acid expression in different transforming virus isolates.

Journal of virology ·Vol. 18 ·No. 2 ·1976-05-00 ·Pages 491-503

Parks WP, Howk RS, Anisowicz A, Scolnick EM

Abstract

The viral polypeptides and viral RNA present in cells transformed by various replication-defective type C viruses derived from Maloney murine leukemia virus were examined. Different portions of the Maloney type C viral genome were retained in the different transforming viruses, thus providing an opportunity for deletion mapping of the Moloney type C genome. DNA transcripts were prepared that are complementary to three distinct nonoverlapping portions of the Moloney viral geonome. Based on an anlysis of the polypeptides produced in the different transformed cells, one complementary DNA apparently respresents sequences coding for Moloney gp70; one complementary DNA represents a region of the Moloney genome common to all of the transforming viruses examined, and one complementary DNA represents the sequences for p30, p15, p10,12. A partial map of the different replication-defective transforming viruses is suggested.

MeSH Terms
Antigens, Viral Base Sequence Cell Line Cell Transformation, Neoplastic Chromosome Mapping DNA, Viral/analysis Genetic Linkage Moloney murine leukemia virus/analysis,growth & development,immunology Peptides/analysis RNA, Viral/analysis Retroviridae/analysis,growth & development,immunology Transcription, Genetic Viral Proteins/analysis,immunology Virus Replication
Chemicals
Antigens, Viral DNA, Viral Peptides RNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Parks W P
Howk R S
Anisowicz A
Scolnick E M
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43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.18.2.491-503.1976
Published
1976-05-00
Pages
491-503
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515574
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.18.2.491-503.1976 PMC Full Text View journal details
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