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PMID: 6308561 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of genomic mouse DNA clones containing sequences homologous to tRNAs and 5S rRNA.

Nucleic acids research ·Vol. 11 ·No. 14 ·1983-07-25 ·Pages 4809-21

Hu JC, Cote BD, Lund E, Dahlberg JE

Abstract

We have cloned and characterized three fragments of Balb/c mouse DNA which hybridize to mouse cell tRNAs. Fractionation of the tRNAs which hybridize to these clones reveals that two of the clones, lambda Mt-4A and lambda Mt-6A hybridize to only one or two tRNAs, while one clone, lambda Mt-4B, hybridizes to at least seven tRNAs. Two of the tRNAs were identified as tRNAProCCG and tRNAGlyGGA, and others have been identified as tRNAs which are selectively encapsidated into virions of murine leukemia virus and avian reticuloendotheliosis virus. The DNA sequences of putative genes for tRNAProCCG and tRNAGlyGGA, plus flanking regions, were determined. A clone of Balb/c mouse DNA which selectively hybridized to 5S rRNA was also isolated and partially characterized.

MeSH Terms
Animals Cloning, Molecular Coliphages/genetics DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Escherichia coli/genetics Mice Mice, Inbred BALB C Molecular Weight Nucleic Acid Conformation Nucleic Acid Hybridization Plasmids RNA, Ribosomal/genetics RNA, Transfer/genetics
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hu J C
Cote B D
Lund E
Dahlberg J E
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.14.4809
Published
1983-07-25
Pages
4809-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326087
Subset
IM
Grants
NIGMS NIH HHS · 5T32 GM07213-003 0221 · United States
NCI NIH HHS · CA-09075 · United States
NCI NIH HHS · CA15166-07 · United States
Databases
GENBANK
X01592, X01593
External Links
PubMed source DOI: 10.1093/nar/11.14.4809 PMC Full Text View journal details
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