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PMID: 6273815 Published · ppublish English Journal Article

Isolation and characterization of cloned rat DNA fragment carrying tRNA genes.

Nucleic acids research ·Vol. 9 ·No. 22 ·1981-11-25 ·Pages 5965-78

Lasser-Weiss M, Bawnik N, Rosen A, Sarid S, Daniel V

Abstract

A rat genomic library was screened for tRNA genes with an unfractionated rat liver tRNA probe. About 70 clones containing tRNA genes were detected per rat genome. The organization of tRNA genes in five clones was analyzed by restriction endonuclease digestion, RNA-DNA hybridization and in vitro transcription with nuclear extracts from Xenopus oocytes. Evidence is presented suggesting that tRNA genes are distributed in the rat genome in small clusters spanning 1 to 2 kb and interspersed with large regions (minimum 8 to 20 kb) of non tRNA-coding DNA. The tRNA gene clusters were found to contain the sequences for a variety of tRNA species. Genes for a single isoacceptor, were found in more than one clone. The detailed study of one clone shows the repetition of a cluster of four tRNA sequences at a distance of about 8 kb. The arrangement of tRNA genes in rat appears to follow the irregular pattern of tRNA gene organization previously reported in Drosophila and Xenopus.

MeSH Terms
Animals Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Liver/metabolism Nucleic Acid Hybridization RNA, Transfer/genetics Rats Transcription, Genetic Xenopus
Chemicals
DNA, Recombinant RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lasser-Weiss M
Bawnik N
Rosen A
Sarid S
Daniel V
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.22.5965
Published
1981-11-25
Pages
5965-78
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327577
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.22.5965 PMC Full Text View journal details
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