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PMID: 6308668 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two nonallelic tRNAiMet genes are located in the p23 leads to q12 region of human chromosome 6.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 16 ·1983-08-00 ·Pages 5027-31

Naylor SL, Sakaguchi AY, Shows TB, Grzeschik KH, Holmes M, Zasloff M

Abstract

Two nonallelic human tRNAiMet genes were assigned to chromosome 6 by filter hybridization of DNA from human-rodent somatic cell hybrids by using probes containing unique sequences from the regions flanking each tRNAiMet gene. These unique sequence probes thus allowed each tRNAiMet gene to be analyzed individually in cell hybrids. Both tRNAiMet genes segregated in the hybrid cells with the chromosome 6 enzyme markers, soluble malic enzyme and the mitochondrial form of superoxide dismutase, and also with a karyotypically normal chromosome 6. By using hybrid clones containing translocations that divide chromosome 6 into five segments, both tRNAiMet genes were assigned to the p23 leads to q12 region. These results raise the possibility that other tRNAiMet genes may be syntenic with the two described in this study and illustrate the utility of using unique flanking sequences to identify members of a multigene family.

MeSH Terms
Alleles Cell Line Chromosomes, Human, 6-12 and X DNA Restriction Enzymes Enzymes/genetics Genes Humans Nucleic Acid Hybridization RNA, Transfer, Amino Acyl/genetics
Chemicals
Enzymes RNA, Transfer, Amino Acyl tRNA(m)(Met), methionine- DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Naylor S L
Sakaguchi A Y
Shows T B
Grzeschik K H
Holmes M
Zasloff M
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.16.5027
Published
1983-08-00
Pages
5027-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384181
Subset
IM
Grants
NIGMS NIH HHS · GM 20454 · United States
NICHD NIH HHS · HD 05196 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.16.5027 PMC Full Text View journal details
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