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PMID: 6308670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 16 ·1983-08-00 ·Pages 5080-4

van der Werf S, Wychowski C, Bruneau P, Blondel B, Crainic R, Horodniceanu F, Girard M

Abstract

Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the beta-lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-beta-lactamase fusion protein that reacted with antibodies raised against poliovirus capsid polypeptide VP1 and with a monoclonal poliovirus type 1 neutralizing antibody, C3. Deletions of various lengths were generated within the VP1 sequence. The hybrid proteins expressed by the deleted plasmids did not react any more with C3 when the region of VP1 amino acids 95-110 (poliovirus nucleotides 2,754-2,806) was deleted. Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid polypeptide.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Antigen-Antibody Complex Base Sequence DNA/analysis DNA Restriction Enzymes Escherichia coli/genetics Mice Plasmids Poliovirus/genetics Viral Proteins/genetics,immunology Viral Structural Proteins
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Viral Proteins Viral Structural Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
van der Werf S
Wychowski C
Bruneau P
Blondel B
Crainic R
Horodniceanu F
Girard M
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.16.5080
Published
1983-08-00
Pages
5080-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384192
Subset
IM
Databases
GENBANK
K00947
External Links
PubMed source DOI: 10.1073/pnas.80.16.5080 PMC Full Text View journal details
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