Effect of heptakis (2,6-O-dimethyl) beta-cyclodextrin on the production of pertussis toxin by Bordetella pertussis.

Infection and immunity ·Vol. 41 ·No. 3 ·1983-09-00 ·Pages 1138-43

Imaizumi A, Suzuki Y, Ono S, Sato H, Sato Y

Abstract

The effect of heptakis (2,6-O-dimethyl) beta-cyclodextrin (Me beta CD) on the production of pertussis toxin was evaluated. The addition of Me beta CD to the medium stimulated cell growth and pertussis toxin production. Me beta CD enhanced pertussis toxin production 100 times more in synthetic media, such as Stainer-Scholte medium (D. W. Stainer and M. J. Scholte, J. Gen. Microbiol. 63:211-220), than in Me beta CD-free medium in 2-day shake cultures. Maximum production of pertussis toxin was estimated as 50 mg of protein per liter of culture broth both by in vitro and in vivo assays. Purified toxin was demonstrated to be biochemically and biologically identical to the toxin produced in Me beta CD-free static cultures.

MeSH Terms

Bacterial Toxins/analysis,biosynthesis Bordetella pertussis/drug effects,metabolism Culture Media/pharmacology Cyclodextrins/pharmacology Dextrins/pharmacology Starch/pharmacology Stimulation, Chemical Time Factors beta-Cyclodextrins

Chemicals

Bacterial Toxins Culture Media Cyclodextrins Dextrins beta-Cyclodextrins heptakis(2,6-O-dimethyl)beta-cyclodextrin Starch

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Imaizumi A

Suzuki Y

Ono S

Sato H

Sato Y

References (13)

13 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.41.3.1138-1143.1983

Published

1983-09-00

Pages

1138-43

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC264618

Subset

External Links

PubMed source DOI: 10.1128/iai.41.3.1138-1143.1983 PMC Full Text View journal details

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Effect of heptakis (2,6-O-dimethyl) beta-cyclodextrin on the production of pertussis toxin by Bordetella pertussis.

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