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PMID: 6309662 Published · ppublish English Journal Article

Serological response to filamentous hemagglutinin and lymphocytosis-promoting toxin of Bordetella pertussis.

Infection and immunity ·Vol. 41 ·No. 3 ·1983-09-00 ·Pages 1150-6

Burstyn DG, Baraff LJ, Peppler MS, Leake RD, St Geme J, Manclark CR

Abstract

Serum antibody responses to the filamentous hemagglutinin and the lymphocytosis-promoting toxin of Bordetella pertussis after vaccination with diphtheria and tetanus toxoids and pertussis vaccine, adsorbed, were assayed by using the enzyme-linked immunosorbent assay. The effect of early immunization, during the first week of life, on the antibody response also was determined. After vaccination, immunoglobulin G (IgG) and IgM directed against both the filamentous hemagglutinin and the lymphocytosis-promoting toxin were detected. Generally, antibody titers increased with subsequent injections and the age of the children. Maternal antibodies against filamentous hemagglutinin and lymphocytosis-promoting toxin were detected in cord blood. The ability of an infant to produce serum IgG anti-lymphocytosis-promoting toxin after vaccination with pertussis vaccine was inversely related to the cord blood serum IgG anti-lymphocytosis-promoting toxin titer at birth. A good antibody response was observed in infants with low cord blood titers, and a poor antibody response was seen in infants with high cord blood values. The IgM anti-lymphocytosis-promoting toxin response was good in groups with both low and high cord blood titer, with no significant difference observed between the two groups. No IgA anti-lymphocytosis-promoting toxin or IgA anti-filamentous hemagglutinin titers were observed in vaccines. IgA antibodies were observed in convalescent sera from two adults and may be presumptive evidence of infection with B. pertussis.

MeSH Terms
Antibodies, Bacterial/analysis Bacterial Toxins/immunology Bordetella pertussis/immunology Diphtheria Toxoid/administration & dosage Enzyme-Linked Immunosorbent Assay Hemagglutinins/immunology Humans Immunization Immunoglobulin A/analysis Immunoglobulin G/analysis Immunoglobulin M/analysis Infant Infant, Newborn Lymphocytosis/chemically induced Pertussis Vaccine/administration & dosage Tetanus Toxoid/administration & dosage
Chemicals
Antibodies, Bacterial Bacterial Toxins Diphtheria Toxoid Hemagglutinins Immunoglobulin A Immunoglobulin G Immunoglobulin M Pertussis Vaccine Tetanus Toxoid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Burstyn D G
Baraff L J
Peppler M S
Leake R D
St Geme J
Manclark C R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.41.3.1150-1156.1983
Published
1983-09-00
Pages
1150-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264620
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.41.3.1150-1156.1983 PMC Full Text View journal details
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