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PMID: 6310504 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromosomal arrangement of leghemoglobin genes in soybean.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5541-53

Lee JS, Brown GG, Verma DP

Abstract

A cluster of four different leghemoglobin (Lb) genes was isolated from AluI-HaeIII and EcoRI genomic libraries of soybean in a set of overlapping clones which together include 45 kilobases (kb) of contiguous DNA. These four genes, including a pseudogene, are present in the same orientation and are arranged in the order: 5'-Lba-Lbc1-Lb psi-Lbc3-3'. The intergenic regions average 2.5 kb. In addition to this main Lb locus, there are other Lb genes which do not appear to be contiguous to this locus. A sequence probably common to the 3' region of Lb loci was found flanking the Lbc3 gene. The 3' flanking region of the main Lb locus also contains a sequence that appears to be expressed more abundantly in root tissue. Another sequence which is primarily expressed in root and leaf is found 5' to two Lb loci. Overall, the main leghemoglobin locus is similar in structure to the mammalian globin gene loci.

MeSH Terms
Base Sequence Biological Evolution Chromosomes/physiology DNA Restriction Enzymes Genes Hemeproteins/genetics Leghemoglobin/genetics Nucleic Acid Hybridization Plants/genetics Soybeans
Chemicals
Hemeproteins Leghemoglobin DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee J S
Brown G G
Verma D P
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26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.16.5541
Published
1983-08-25
Pages
5541-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326296
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.16.5541 PMC Full Text View journal details
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