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PMID: 6897566 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human globin psi B2 is not a globin-related sequence.

Nucleic acids research ·Vol. 10 ·No. 23 ·1982-12-11 ·Pages 7809-18

Shen SH, Smithies O

Abstract

We have determined the complete nucleotide sequence of 3.4 kilobase pairs of DNA covering the region of the human beta globin gene cluster where a human globin-related sequence psi beta 2 was thought to lie (Fritsch, Lawn, and Maniatis (1980) Cell 19, 959-972). Analysis of the resulting data reveals no evidence for any globin-related sequences in this region. The region does, however, contain several stretches of poly (dA-dT). We have confirmed the observations of Fritsch et al. that DNA from the psi beta 2 region hybridizes to the poly (dA-dT)-tailed human fetal globin cDNA plasmid, pJW151 (Wilson et al., (1978) Nucl. Acids Res. 5, 563-581) under conditions of low stringency, but we find that this hybridization is abolished by the addition of poly(rA). We conclude that psi beta 2 is not a globin pseudogene, and that the earlier investigators were probably misled by hybridization between the poly (dA-dT) stretches within the psi beta 2 region and the tails used in constructing the cDNA plasmid.

MeSH Terms
Base Sequence DNA/genetics,metabolism Female Fetus Genes Globins/genetics Humans Nucleic Acid Hybridization Plasmids Pregnancy
Chemicals
Globins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shen S H
Smithies O
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.23.7809
Published
1982-12-11
Pages
7809-18
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327047
Subset
IM
Grants
NIADDK NIH HHS · AM20120 · United States
NIGMS NIH HHS · GM20069 · United States
Databases
GENBANK
J00093, J00094, J00096, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179, K01239, K01890, K02544, M18047, M19067, X00423
External Links
PubMed source DOI: 10.1093/nar/10.23.7809 PMC Full Text View journal details
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