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PMID: 6310522 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High spontaneous mutation rate of Rous sarcoma virus demonstrated by direct sequencing of the RNA genome.

Nucleic acids research ·Vol. 11 ·No. 17 ·1983-09-10 ·Pages 5953-67

Darlix JL, Spahr PF

Abstract

Direct and extensive sequencing of RSV RNA genome is reported. More than 10,000 nt of the T1 RNase resistant RSV RNA fragments (1) have been sequenced and shown to cover 3900 nt of RSV genome. The frequent sequence variations found indicate that RSV supports a very high incidence of spontaneous mutations in the course of replication, one very probable cause of the genetic diversity among the avian retroviruses. Sequences of the structured RSV RNAs allowed us also to precisely characterize the structured domains of the retroviral genome and show that the src gene is not structured.

MeSH Terms
Amino Acid Sequence Animals Avian Sarcoma Viruses/genetics Base Sequence Chick Embryo Fibroblasts Genes, Viral Kinetics Mutation RNA, Viral/genetics,isolation & purification Ribonuclease T1 Viral Proteins/genetics Virion/genetics
Chemicals
RNA, Viral Viral Proteins Ribonuclease T1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Darlix J L
Spahr P F
References (32)
32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.17.5953
Published
1983-09-10
Pages
5953-67
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326329
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.17.5953 PMC Full Text View journal details
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