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PMID: 6313617 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a Rhodospirillum rubrum plasmid: loss of photosynthetic growth in plasmidless strains.

Journal of bacteriology ·Vol. 156 ·No. 2 ·1983-11-00 ·Pages 737-42

Kuhl SA, Nix DW, Yoch DC

Abstract

A single plasmid of 55 kilobases was found in crude cell lysates of each of nine strains of Rhodospirillum rubrum. Restriction endonuclease analysis showed identical fragment patterns with a given nuclease for all plasmids except one, for which an additional EcoRI site was observed. Elimination of the plasmid required that the cells be passaged several times in 25 mM calcium-containing medium, followed by at least two passages under photosynthetic growth conditions in low-calcium medium before treatment with ethyl methanesulfonate. The resulting plasmidless mutants only grew aerobically and were all incapable of pigment formation and photosynthetic growth, suggesting that plasmid DNA is required for photosynthetic competence in R. rubrum.

MeSH Terms

Base Composition Calcium/pharmacology DNA Restriction Enzymes Ethyl Methanesulfonate/pharmacology Photosynthesis Plasmids Rhodospirillum rubrum/drug effects,genetics,metabolism

Chemicals

Ethyl Methanesulfonate DNA Restriction Enzymes Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kuhl S A

Nix D W

Yoch D C

References (13)

13 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.156.2.737-742.1983

Published

1983-11-00

Pages

737-42

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC217890

Subset

Grants

NCRR NIH HHS · 5 SO7 RR07160 · United States

NIGMS NIH HHS · GM-32183-04 · United States

External Links

PubMed source DOI: 10.1128/jb.156.2.737-742.1983 PMC Full Text View journal details

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Characterization of a Rhodospirillum rubrum plasmid: loss of photosynthetic growth in plasmidless strains.

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