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PMID: 6314249 Published · ppublish English Journal Article

The complete nucleotide sequence of the gene coding for diphtheria toxin in the corynephage omega (tox+) genome.

Nucleic acids research ·Vol. 11 ·No. 19 ·1983-10-11 ·Pages 6589-95

Ratti G, Rappuoli R, Giannini G

Abstract

A segment of corynephage omega (tox+) DNA, containing the gene for diphtheria toxin (tox) was fragmented with restriction enzymes and the fragments cloned into M13 vectors for nucleotide sequence determination. A long open reading frame was shown to encode the tox gene by comparing the predicted amino acid sequence with that of peptides derived from the mature toxin molecule. Analysis of the nucleotide sequence shows RNA polymerase and ribosome binding signals preceding a GTG codon in the open reading frame: if this is the correct starting signal for translation, then a 25 amino acid signal peptide can be predicted for the toxin molecule.

MeSH Terms
Amino Acid Sequence Bacteriophages/genetics Base Sequence Corynebacterium diphtheriae/genetics DNA Restriction Enzymes DNA-Directed RNA Polymerases/metabolism Diphtheria Toxin/genetics Genes Genes, Bacterial Genes, Viral Transcription, Genetic
Chemicals
Diphtheria Toxin DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ratti G
Rappuoli R
Giannini G
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.19.6589
Published
1983-10-11
Pages
6589-95
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326401
Subset
IM
Databases
GENBANK
M19546
External Links
PubMed source DOI: 10.1093/nar/11.19.6589 PMC Full Text View journal details
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