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PMID: 6315414 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Formation of Z-DNA in negatively supercoiled plasmids is sensitive to small changes in salt concentration within the physiological range.

The EMBO journal ·Vol. 2 ·No. 5 ·1983-00-00 ·Pages 649-55

Azorin F, Nordheim A, Rich A

Abstract

Negative supercoiling of the plasmid pBR322 with or without an insert of (dG-dC)n induces the formation of Z-DNA as measured by the binding of antibodies specific for Z-DNA. Increasing the concentration of Na+ (or K+) is shown to inhibit the B to Z-DNA conversion. This may be due to the effect of the cation on the B-Z junction. Using the data for B to Z-DNA conversion of the (dG-dC)n inserts, we have estimated the free energy change per base pair as well as the energy of the B-Z junction. In pBR322, a 14-bp segment [CACGGGTGCGCATG] is believed to form Z-DNA at bacterial negative superhelical densities under salt conditions which are similar to those found in vivo.

MeSH Terms

DNA/metabolism DNA Topoisomerases, Type I/metabolism DNA, Superhelical/metabolism Nucleic Acid Conformation Osmolar Concentration Plasmids Sodium Chloride/pharmacology

Chemicals

DNA, Superhelical Sodium Chloride DNA DNA Topoisomerases, Type I

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Azorin F

Nordheim A

Rich A

References (26)

26 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1983-00-00

Pages

649-55

Language

English

Region

England

NLM ID

8208664

PMCID

PMC555164

Subset

External Links

PubMed source PMC Full Text View journal details

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Formation of Z-DNA in negatively supercoiled plasmids is sensitive to small changes in salt concentration within the physiological range.

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