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PMID: 6316273 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The structure of rat 28S ribosomal ribonucleic acid inferred from the sequence of nucleotides in a gene.

Nucleic acids research ·Vol. 11 ·No. 22 ·1983-11-25 ·Pages 7819-31

Chan YL, Olvera J, Wool IG

Abstract

The nucleotide sequence of a rat 28S rRNA gene was determined. The 28S rRNA encoded in the gene contains 4718 nucleotides and the molecular weight estimated from the sequence is 1.53 x 10(6). The guanine and cytosine content is 67%. The sequence of rat 28S rRNA diverges appreciably from that of Saccharomyces carlsbergensis 26S rRNA (about 50% identity), but more closely approximates that of Xenopus laevis 28S rRNA (about 75% identity). Rat 28S rRNA is larger than the analogous nucleic acids from yeast (3393 nucleotides) and X, laevis (4110 nucleotides) ribosomes. The additional bases are inserted in specific regions and tend to be rich in guanine and cytosine. 5.8S rRNA can interact with 28S rRNA by extensive hydrogen bonding at two sites near the 5' end of the latter.

MeSH Terms
Animals Base Sequence Biological Evolution DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Genes RNA, Ribosomal/genetics Rats Saccharomyces/genetics Saccharomyces cerevisiae/genetics Species Specificity Xenopus
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chan Y L
Olvera J
Wool I G
References (34)
34 references, click to expand
  1. Characterization of a new low molecular weight RNA in HeLa cell ribosomes.
    J Mol Biol. 1968 May 14;33(3):609-23 PMID: 5700415
  2. Molecular weights of ribosomal RNA in relation to evolution.
    J Mol Biol. 1968 Dec;38(3):355-65 PMID: 5718556
  3. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  4. Processing of 45 s nucleolar RNA.
    J Mol Biol. 1970 Jan 28;47(2):169-78 PMID: 4314037
  5. Size heterogeneity of the large ribosomal subunits and conservation of the small subunits in eucaryote evolution.
    Biochim Biophys Acta. 1972 Nov 9;281(4):597-624 PMID: 4569289
  6. Formation of active hybrid 80-S particles from subunits of pea seedlings and mammalian liver ribosomes.
    Biochim Biophys Acta. 1972 Nov 9;281(4):625-42 PMID: 4653130
  7. A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli.
    Biochem Biophys Res Commun. 1973 Oct 15;54(4):1451-9 PMID: 4585292
  8. Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid.
    J Biol Chem. 1975 Nov 25;250(22):8591-7 PMID: 171258
  9. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  10. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  11. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  12. Extensive homologies between the methylated nucleotide sequences in several vertebrate ribosomal ribonucleic acids.
    Biochem J. 1978 Mar 1;169(3):531-42 PMID: 417718
  13. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  14. The rat serum albumin gene: analysis of cloned sequences.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3256-60 PMID: 158758
  15. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  16. Computer analysis of nucleic acids and proteins.
    Methods Enzymol. 1980;65(1):595-609 PMID: 6154871
  17. A 5.8 S rRNA-like sequence in prokaryotic 23 S rRNA.
    FEBS Lett. 1980 Oct 6;119(2):212-4 PMID: 6159235
  18. The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1980 Dec 11;8(23):5779-94 PMID: 7008030
  19. Nucleotide sequence through the 18S-28S intergene region of a vertebrate ribosomal transcription unit.
    Nucleic Acids Res. 1980 Dec 20;8(24):5993-6005 PMID: 6258158
  20. Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequence.
    Nature. 1981 May 21;291(5812):205-8 PMID: 7015146
  21. The nucleotide sequence at the 3'-end of Neurospora crassa 25S-rRNA and the location of a 5.8S-rRNA binding site.
    Nucleic Acids Res. 1981 Mar 11;9(5):1111-21 PMID: 6453330
  22. Sequence homologies between eukaryotic 5.8S rRNA and the 5' end of prokaryotic 23S rRNa: evidences for a common evolutionary origin.
    Nucleic Acids Res. 1981 Jun 25;9(12):2913-32 PMID: 7024907
  23. Secondary structure model for 23S ribosomal RNA.
    Nucleic Acids Res. 1981 Nov 25;9(22):6167-89 PMID: 7031608
  24. The primary and secondary structure of yeast 26S rRNA.
    Nucleic Acids Res. 1981 Dec 21;9(24):6935-52 PMID: 7335496
  25. The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25 S rRNA gene from Saccharomyces cerevisae.
    Nucleic Acids Res. 1981 Dec 21;9(24):6953-8 PMID: 6460984
  26. The nucleotide sequence of 5S RNA from rat liver ribosomes.
    J Biochem. 1982 Jan;91(1):363-7 PMID: 6175626
  27. Isolation and characterization of rat ribosomal DNA clones.
    Gene. 1982 Jan;17(1):75-7 PMID: 6281138
  28. Cloning and determination of the transcription termination site of ribosomal RNA gene of the mouse.
    Nucleic Acids Res. 1982 Mar 25;10(6):1963-79 PMID: 6281727
  29. Enzymatic and chemical structure mapping of mouse 28S ribosomal ribonucleic acid contacts in 5.8S ribosomal ribonucleic acid.
    Biochemistry. 1982 May 11;21(10):2320-9 PMID: 7093191
  30. Independent binding sites in mouse 5.8S ribosomal ribonucleic acid for 28S ribosomal ribonucleic acid.
    Biochemistry. 1982 May 11;21(10):2329-35 PMID: 6284197
  31. The site of action of alpha-sarcin on eukaryotic ribosomes. The sequence at the alpha-sarcin cleavage site in 28 S ribosomal ribonucleic acid.
    J Biol Chem. 1982 Aug 10;257(15):9054-60 PMID: 7047533
  32. Nucleotide sequence of the region between the 18S rRNA sequence and the 28S rRNA sequence of rat ribosomal DNA.
    Nucleic Acids Res. 1982 Jun 25;10(12):3667-80 PMID: 6287418
  33. Sequence and secondary structure of mouse 28S rRNA 5'terminal domain. Organisation of the 5.8S-28S rRNA complex.
    Nucleic Acids Res. 1982 Sep 11;10(17):5273-83 PMID: 6292836
  34. Location of 5.8 S rRNA contact sites in 28 S rRNA and the effect of alpha-sarcin on the association of 5.8 S rRNA with 28 S rRNA.
    J Biol Chem. 1983 Jan 10;258(1):333-8 PMID: 6294104
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.22.7819
Published
1983-11-25
Pages
7819-31
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326539
Subset
IM
Grants
NCI NIH HHS · CA 19265 · United States
NIGMS NIH HHS · GM 21769 · United States
Databases
GENBANK
K01591, X01069
External Links
PubMed source DOI: 10.1093/nar/11.22.7819 PMC Full Text View journal details
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