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PMID: 6318080 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Co-amplification of rRNA genes with CAD genes in N-(phosphonacetyl)-L-aspartate-resistant Syrian hamster cells.

Molecular and cellular biology ·Vol. 3 ·No. 11 ·1983-11-00 ·Pages 2066-75

Wahl GM, Vitto L, Rubnitz J

Abstract

The amplified CAD genes in N-(phosphonacetyl)-L-aspartate (PALA)-resistant Syrian hamster cells are located in an expanded chromosomal region emanating from the site of the wild-type gene at the tip of the short arm of chromosome B-9. The terminus of B-9 in PALA-sensitive cells contains a cluster of rRNA genes (i.e., a nucleolus organizer, rDNA). We have used a molecular clone containing sequences complementary to Syrian hamster 28S rRNA to investigate whether rDNA is coamplified with CAD genes in the PALA-resistant mutants. In situ hybridization of this probe to metaphase chromosomes demonstrates that rDNA and CAD genes do coamplify in two independently isolated PALA-resistant mutants. The tight linkage of CAD and rDNA genes was demonstrated by their coordinate translocation from B-9 to the end of the long arm of chromosome C-11 in one mutant. Blot hybridization studies substantiate the in situ hybridization results. Both types of analysis indicate that only one or two rDNA genes, on the average, are coamplified per CAD gene. The data are consistent with the model that unequal exchanges between rDNA genes mediate the amplification of CAD genes in the Syrian hamster mutants that were analyzed.

MeSH Terms
Animals Aspartate Carbamoyltransferase Aspartic Acid/analogs & derivatives,pharmacology Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) Cell Line Chromosomes Cricetinae DNA Restriction Enzymes Dihydroorotase Drug Resistance Gene Amplification Genes Mesocricetus Models, Genetic Multienzyme Complexes Nucleic Acid Hybridization Organophosphorus Compounds/pharmacology Phosphonoacetic Acid/analogs & derivatives,pharmacology Proteins/genetics RNA, Ribosomal/genetics Sister Chromatid Exchange
Chemicals
CAD trifunctional enzyme Multienzyme Complexes Organophosphorus Compounds Proteins RNA, Ribosomal Aspartic Acid sparfosic acid Aspartate Carbamoyltransferase DNA Restriction Enzymes Dihydroorotase Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) Phosphonoacetic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wahl G M
Vitto L
Rubnitz J
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36 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.11.2066-2075.1983
Published
1983-11-00
Pages
2066-75
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC370073
Subset
IM
Grants
NIGMS NIH HHS · GM 27754 · United States
External Links
PubMed source DOI: 10.1128/mcb.3.11.2066-2075.1983 PMC Full Text View journal details
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