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PMID: 6320176 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Template requirements for initiation of phage phi 29 DNA replication in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 1 ·1984-01-00 ·Pages 80-4

García JA, Peñalva MA, Blanco L, Salas M

Abstract

The template requirements for the formation of the phi 29 protein p3-dAMP initiation complex in vitro have been studied. The initiation reaction requires the parental protein p3 but not an intact DNA molecule. Protein p3-containing fragments from the left- or right-hand DNA ends were active as template for formation of the initiation complex provided they had a minimal size: a 26-base-pair-long fragment was active whereas a 10-base-pair-long one was essentially inactive. However, the activity of the latter was restored by ligation of an unspecific DNA sequence. phi 29 DNA internal fragments, as well as denatured phi 29 DNA, were inactive as template for the initiation reaction. The terminal protein-DNA complex isolated from Bacillus phage phi 15 was active in formation of the phi 29 p3-dAMP complex, whereas the protein-DNA complex isolated from Bacillus phage GA-1 or from the pneumococcal phage Cp-1, both with a morphology similar to that of phage phi 29, as well as that obtained from adenovirus, were inactive.

MeSH Terms
Bacillus subtilis/genetics Bacteriophages/genetics Base Sequence DNA Replication DNA Restriction Enzymes DNA, Viral/genetics Deoxyadenine Nucleotides Peptide Initiation Factors Templates, Genetic Viral Proteins/genetics Virus Replication
Chemicals
DNA, Viral Deoxyadenine Nucleotides Peptide Initiation Factors Viral Proteins 2'-deoxy-5'-adenosine monophosphate DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
García J A
Peñalva M A
Blanco L
Salas M
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.1.80
Published
1984-01-00
Pages
80-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344614
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.1.80 PMC Full Text View journal details
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