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PMID: 6321432 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of the replication and stability regions of Agrobacterium tumefaciens plasmid pTAR.

Journal of bacteriology ·Vol. 157 ·No. 3 ·1984-03-00 ·Pages 739-45

Gallie DR, Zaitlin D, Perry KL, Kado CI

Abstract

A 5.4-kilobase region containing the origin of replication and stability maintenance of the 44-kilobase Agrobacterium tumefaciens plasmid pTAR has been mapped and characterized. Within this region is a 1.3-kilobase segment that is capable of directing autonomous replication. The remaining segment contains the stability locus for maintenance of pTAR during nonselective growth. Approximately 35% of pTAR shares sequence homology with pAg119, a 44-kilobase cryptic plasmid in grapevine strain 1D1119. However, no homology was detected between pTAR DNA and several Ti plasmids or several other small cryptic plasmids in many A. tumefaciens strains. A recombinant plasmid containing the origin of replication and stability maintenance region of pTAR was compatible with pTiC58, pTi15955, and pTi119 and incompatible with pAg119. A new compatibility group, Inc Ag-1, is discussed.

MeSH Terms
Base Sequence DNA Replication DNA Restriction Enzymes DNA, Bacterial Nucleic Acid Hybridization Plasmids Rhizobium/genetics
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gallie D R
Zaitlin D
Perry K L
Kado C I
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27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.3.739-745.1984
Published
1984-03-00
Pages
739-45
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215320
Subset
IM
Grants
NCI NIH HHS · CA-11526 · United States
External Links
PubMed source DOI: 10.1128/jb.157.3.739-745.1984 PMC Full Text View journal details
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