Repetitive DNA of Candida albicans: nuclear and mitochondrial components.

Journal of bacteriology ·Vol. 157 ·No. 3 ·1984-03-00 ·Pages 918-24

Wills JW, Lasker BA, Sirotkin K, Riggsby WS

Abstract

We report the isolation and analysis of the rapidly reassociating DNA of the pathogenic, dimorphic fungus Candida albicans. Minicot analysis of whole-cell repetitive DNA suggested that a significant portion of this component was mitochondrial DNA. Genomic blot hybridizations in which radioactive whole-cell repetitive DNA was used as a probe revealed eight major EcoRI bands in the molecular weight range resolved by the gel system used. Isolation and analysis of high-purity mitochondrial DNA have shown that five of these bands are of mitochondrial origin. The remaining three bands are of nuclear origin and represent repetitive sequences that are found in the nuclear genome. Attempts to isolate nuclear DNA that was completely free of mitochondrial DNA contamination were unsuccessful.

MeSH Terms

Candida albicans/genetics,ultrastructure Cell Fractionation Cell Nucleus/analysis DNA Restriction Enzymes DNA, Fungal/analysis,isolation & purification DNA, Mitochondrial/analysis,isolation & purification Deoxyribonuclease EcoRI Nucleic Acid Hybridization Nucleic Acid Renaturation Repetitive Sequences, Nucleic Acid

Chemicals

DNA, Fungal DNA, Mitochondrial DNA Restriction Enzymes Deoxyribonuclease EcoRI

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wills J W

Lasker B A

Sirotkin K

Riggsby W S

References (31)

31 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.157.3.918-924.1984

Published

1984-03-00

Pages

918-24

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215346

Subset

External Links

PubMed source DOI: 10.1128/jb.157.3.918-924.1984 PMC Full Text View journal details

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Repetitive DNA of Candida albicans: nuclear and mitochondrial components.

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