Home LiteratureArticle Details
PMID: 6248732 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Anatomy of mitochondrial DNA from Paramecium aurelia.

Molecular & general genetics : MGG ·Vol. 178 ·No. 3 ·1980-00-00 ·Pages 499-510

Cummings DJ, Maki RA, Conlon PJ, Laping J

Abstract

The linear genome of mitochondrial DNA from four species of Paramecium aurelia was investigated with respect to restriction endonuclease fragments, location and number of ribosomal RNA genes, and interspecies EcoRI and HindIII fragment homologies. One copy of each of the rRNA genes was found in all four species and the 14s and 20s rRNA genes were separated by at least 3,000 bp. R-Loop analysis of the 20s rRNA gene did not reveal the presence of an intervening sequence. Interspecies homology studies showed species 1, 5, and 7 to have a high degree of homology but species 4 was less than 50% homologous to species 1 mt DNA. For all four species, rRNA genes showed good homology indicating that these DNA sequences are highly conserved, even between species having many non-homologous regions. A major region of DNA which displayed little homology between species 1 and 4 was that fragment containing sequences essential for initiation of DNA replication.

MeSH Terms
Animals Base Sequence Chromosome Mapping DNA Restriction Enzymes/metabolism DNA, Mitochondrial/analysis,genetics Genes Microscopy, Electron Nucleic Acid Conformation Nucleic Acid Hybridization Paramecium/genetics RNA, Ribosomal/genetics Species Specificity
Chemicals
DNA, Mitochondrial RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cummings D J
Maki R A
Conlon P J
Laping J
References (31)
31 references, click to expand
  1. Physicochemical properties of complexes between deoxyribonucleic acid and antibiotics which affect ribonucleic acid synthesis (actinomycin, daunomycin, cinerubin, nogalamycin, chormomycin, mithramycin, and olivomycin).
    Biochemistry. 1966 Jan;5(1):236-44 PMID: 4161040
  2. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.
    J Biol Chem. 1977 Nov 25;252(22):8118-25 PMID: 334774
  3. A model for replication of the ends of linear chromosomes.
    Nucleic Acids Res. 1976 Nov;3(11):3167-71 PMID: 188017
  4. Characterization of mitochondrial and cytoplasmic ribosomes from Paramecium aurelia.
    J Cell Biol. 1977 Apr;73(1):139-48 PMID: 856828
  5. Conservation of the sequence and position of the ribosomal RNA genes in Tetrahymena pyriformis mitochondrial DNA.
    Biochim Biophys Acta. 1978 Nov 21;521(1):187-97 PMID: 102354
  6. Structure and replication of mitochondrial DNA from Paramecium aurelia.
    J Mol Biol. 1975 Oct 5;97(4):593-609 PMID: 1185783
  7. Free ribosomal RNA genes in Paramecium are tandemly repeated.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3312-6 PMID: 277929
  8. Characterization of mitochondrial DNA from Paramecium aurelia with EcoRI and Hae II restriction endonucleases.
    Plasmid. 1977 Nov;1(1):106-14 PMID: 618181
  9. The organization of ribosomal RNA genes in the mitochondrial DNA of Tetrahymena pyriformis strain ST.
    Biochim Biophys Acta. 1978 Nov 21;521(1):169-86 PMID: 102353
  10. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. I. PURIFICATION OF THE ENZYME AND CHARACTERIZATION OF THE PHOSPHATASE ACTIVITY.
    J Biol Chem. 1964 Jan;239:242-50 PMID: 14114850
  11. Accumulation of replicative intermediates of mitochondrial DNA in Tetrahymena pyriformis grown in ethidium bromide.
    J Cell Biol. 1974 May;61(2):383-97 PMID: 4208072
  12. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.
    Cell. 1977 Feb;10(2):177-92 PMID: 402221
  13. Observations on two mitochondrial phenotypes in single paramecium cells.
    Exp Cell Res. 1972 Jan;70(1):223-6 PMID: 5008400
  14. An insert in the single gene for the large ribosomal RNA in yeast mitochondrial DNA.
    Nature. 1978 Sep 28;275(5678):336-8 PMID: 357991
  15. Hybridization of RNA to double-stranded DNA: formation of R-loops.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2294-8 PMID: 781674
  16. Mitochondrial DNA from Podospora anserina. I. Isolation and characterization.
    Mol Gen Genet. 1979 Mar 27;171(3):229-38 PMID: 286867
  17. An analysis by electron microscopy of intermediates in the replication of linear Tetrahymena mitochondrial DNA.
    Biochim Biophys Acta. 1974 Sep 13;361(3):266-76 PMID: 4214118
  18. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  19. The organization of genes in yeast mitochondrial DNA. I. The genes for large and small ribosomal RNA are far apart.
    Biochem Biophys Res Commun. 1975 Jul 22;65(2):699-707 PMID: 1148012
  20. Mitochondrial DNA replication in Paramecium aurelia. Cross-linking of the initiation end.
    J Mol Biol. 1977 Jan 15;109(2):327-44 PMID: 839545
  21. Evidence for semi-conservative replication of mitochondrial DNA from Paramecium aurelia.
    J Mol Biol. 1977 Nov 25;117(1):273-7 PMID: 599567
  22. Organization of tRNA and rRNA genes in N. crassa mitochondria: intervening sequence in the large rRNA gene and strand distribution of the RNA genes.
    Cell. 1979 Jul;17(3):583-95 PMID: 157815
  23. Size and structural variations of mitochondrial DNA.
    Proc Natl Acad Sci U S A. 1968 May;60(1):235-42 PMID: 16591633
  24. Mitochondrial genetics in Paramecium.
    Nature. 1972 Feb 18;235(5338):396-7 PMID: 4551647
  25. Comparative studies on mitochondrial and cytoplasmic ribosomes of Tetrahymena pyriformis.
    J Mol Biol. 1970 Nov 14;53(3):531-56 PMID: 5493283
  26. The use of RNA labeled in vitro with iodine-125 in molecular hybridization experiments.
    Biochim Biophys Acta. 1972 Dec 22;287(3):485-94 PMID: 4629777
  27. Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation.
    Nucleic Acids Res. 1977 Sep;4(9):3055-64 PMID: 410006
  28. Interspecies transfer of mitochondria in Paramecium aurelia.
    Mol Gen Genet. 1976 Jan 16;143(2):197-201 PMID: 1250220
  29. Mitochondrial nucleic acids.
    Annu Rev Biochem. 1972;41:333-76 PMID: 4563438
  30. Encephalomyocarditis virus RNA: variations in polyadenylic acid content and biological activity.
    J Virol. 1976 Aug;19(2):325-30 PMID: 183008
  31. The use of R-looping for structural gene identification and mRNA purification.
    Nucleic Acids Res. 1979 Jun 11;6(7):2483-97 PMID: 379820
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337854
Published
1980-00-00
Pages
499-510
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337854
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2