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PMID: 6322112 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of Ty-lacZ fusions in Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 12 ·No. 3 ·1984-02-10 ·Pages 1627-40

Bowen BA, Fulton AM, Tuite MF, Kingsman SM, Kingsman AJ

Abstract

We have determined the nucleotide sequence of about 520 bp spanning the 5' delta regions (Figure 1) of two Tyl elements. There is an open reading frame running out of the deltas for at least 180 nucleotides into the internal region of each element. The functional significance of these open reading frames has been tested by fusing them to a defective E.coli lacZ gene. Expression of B-galactosidase in yeast transformants containing these fusions shows that Tyl elements contain functional translation signals.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Galactosidases/genetics Genes Genes, Viral Plasmids Saccharomyces cerevisiae/enzymology,genetics beta-Galactosidase/genetics,metabolism
Chemicals
DNA Transposable Elements DNA Restriction Enzymes Galactosidases beta-Galactosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bowen B A
Fulton A M
Tuite M F
Kingsman S M
Kingsman A J
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.3.1627
Published
1984-02-10
Pages
1627-40
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318604
Subset
IM
Databases
GENBANK
X00393, X00394, X02546
External Links
PubMed source DOI: 10.1093/nar/12.3.1627 PMC Full Text View journal details
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