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PMID: 6323767 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and genetic mapping of a herpes simplex virus capsid protein that binds DNA.

Journal of virology ·Vol. 50 ·No. 2 ·1984-05-00 ·Pages 645-8

Braun DK, Batterson W, Roizman B

Abstract

Herpes simplex virus virion protein 19C (VP19C) is a constituent of both unenveloped (nuclear) and enveloped (cytoplasmic) capsids. In this paper we report that 32P-labeled DNA, either supercoiled or linear double stranded, efficiently bound to VP19C electrically transferred from denaturing polyacrylamide gels containing electrophoretically separated proteins from purified capsids. Analyses of the polypeptides specified by herpes simplex virus type 1 X herpes simplex type 2 recombinants with respect to electrophoretic mobility and binding of 32P-labeled DNA indicate that VP19C maps at the same location as infected cell polypeptide 32 and is derived from it.

MeSH Terms
Animals Base Sequence Capsid/genetics,metabolism DNA/metabolism Genes Genes, Viral Protein Binding Simplexvirus/genetics
Chemicals
DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Braun D K
Batterson W
Roizman B
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25 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.2.645-648.1984
Published
1984-05-00
Pages
645-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255696
Subset
IM
Grants
NIAID NIH HHS · 5 T32 PHS AI-07182 · United States
NCI NIH HHS · CA-08494 · United States
NCI NIH HHS · CA-19264 · United States
External Links
PubMed source DOI: 10.1128/JVI.50.2.645-648.1984 PMC Full Text View journal details
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