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PMID: 6324086 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two nuclear DNA binding proteins of Dictyostelium discoideum with a high affinity for poly(dA)-poly(dT).

Nucleic acids research ·Vol. 11 ·No. 23 ·1983-12-10 ·Pages 8473-84

Garreau H, Williams JG

Abstract

Intergenic regions of the Dictyostelium genome contain an extremely high proportion of AT base pairs. Those intergenic regions which have been subjected to nucleotide sequence analysis are predominantly composed of alternating runs of poly(dA) and poly(dT) and there is evidence to suggest that nucleosomes do not form on such sequences. We have identified two nuclear proteins, of molecular weight 70,000 and 74,000 daltons, which bind only to intergenic regions of a cloned Dictyostelium gene. Binding is specifically inhibited in the presence of synthetic poly(dA) - poly (dT) as competitor. These proteins may play some role in the chromosomal organization of intergenic regions in Dictyostelium discoideum.

MeSH Terms

Base Sequence Carrier Proteins/genetics Cell Nucleus/metabolism DNA Restriction Enzymes DNA, Fungal/genetics DNA-Binding Proteins/genetics,metabolism Dictyostelium/genetics Discoidins Fungal Proteins/genetics Genes Genes, Fungal Lectins Molecular Weight Plasmids Poly dA-dT/metabolism Polydeoxyribonucleotides/metabolism Protozoan Proteins

Chemicals

Carrier Proteins DNA, Fungal DNA-Binding Proteins Discoidins Fungal Proteins Lectins Polydeoxyribonucleotides Protozoan Proteins Poly dA-dT DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Garreau H

Williams J G

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24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.23.8473

Published

1983-12-10

Pages

8473-84

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326596

Subset

External Links

PubMed source DOI: 10.1093/nar/11.23.8473 PMC Full Text View journal details

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Two nuclear DNA binding proteins of Dictyostelium discoideum with a high affinity for poly(dA)-poly(dT).

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